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Phosphorothioates

Research Diagnostic Therapeutic
Phosphorothioate analogs in which a non-bridging oxygen has been replaced by sulfur have widespread use in molecular biology. The increased resistance of phosphorothioates against nuclease digestion has prompted the consideration of these molecules for medical purposes.


NameSynthesis scalePurificationReferenceEUR
DNA Phosphorothioates bases0.2 µmolRP-HPLCOL-00414.50
DNA Phosphorothioates bases1.0 µmolRP-HPLCOL-00425.60
DNA Phosphorothioates bases2.5 µmolRP-HPLCOL-004315.00
DNA Phosphorothioates bases5 µmolRP-HPLCOL-004425.00
DNA Phosphorothioates bases10 µmolRP-HPLCOL-004535.00
DNA Phosphorothioates bases35 µmolRP-HPLCOL-004645.00
DNA Phosphorothioates bases50 µmolRP-HPLCOL-004785.00
5' Iodo + 3' phosphorotioate bases0.2 µmolRP-HPLCOL-0048-IODO02281.50
RNA Phosphorotioates0.2 µmolPAGEOL-004928.00
RNA Phosphorotioates1.0 µmolPAGEOL-005031.00
RNA Phosphorotioates2.5 µmolRP-HPLCOL-0051140.00
RNA Phosphorotioates5 µmolRP-HPLCOL-0052350.00
RNA Phosphorotioates10 µmolRP-HPLCOL-0053559.00
Price per incorporation

Quality Control

   MALDI-TOF Mass Spectrometry

Delivery times

   10 Working days

Packaging

   Lyophilised

Shipping conditions

   Room temperature

Storage conditions

   -20 °C to -70 °C
Oligonucleotides are stable in solution at 4 °C for up to 2 weeks. Properly reconstituted material stored at -20 °C should be stable for at least 6 months. Lyophilized DNA (when kept at -20 °C) in a nuclease-free environment should be stable for years.

Phosphorothioate analogs in which a non-bridging oxygen has been replaced by sulfur have widespread use in molecular biology. The increased resistance of phosphorothioates against nuclease digestion has prompted the consideration of these molecules for medical purposes. 

In addition to their value as antisense probes, phosphorothioates have also been used for the site specific attachment of reporter groups at the DNA/RNA backbone and for preventing degradation of oligonucleotides by 3’/5’-exonuclease activities of proofreading DNA polymerases such as Vent and Pfu. Phosphorothioate substituted RNA has been used to study specific RNA-Protein interactions.



Legal notices

   For Research Use only

Leaflets

Product citations


FARIA M. et al., "Phosphoramidate oligonucleotides as potent antisense molecules in cells and in vivo", Nature Biotechnology, vol. 19, n° 1, p. 40-44, January 2001


DE BACKER M. et al., "An antisense-based functional genomics approach for identification of genes critical for growth of Candida albicans", Nature Biotechnology, vol. 19, n° 3, p. 235-241, March 2001


ANGO F. et al., "Agonist-independent activation of metabotropic glutamate receptors by the intracellular protein Homer", Nature, n° 411, p. 962-965, 21 June 2001


VAN HEUSDEN J. et al., "Inhibition of all-TRANS-retinoic acid metabolism by R116010 induces antitumour activity", British Journal of Cancer, vol. 86, n° 4, p. 605-611, 12 February 2002


SKORDIS L. et al., "Bifunctional antisense oligonucleotides provide a trans-acting plicing enhancer that stimulates SMN2 gene expression in paient fibroblasts", PNAS, vol. 100, n° 7, p. 4114-4119, April 2003


GROTE K. et al., "Stretch-inducible Expression of the Angiogenic Factor CCN1 in Vascular Smooth Muscle Cells Is Mediated by Egr-1", Journal of Biological Chemistry, vol. 279, n° 53, p. 55675-55681, December 2004





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