Tth DNA Polymerase is a thermostable enzyme that replicates DNA at 74 °C and exhibits a half-life of 20 minutes at 95 °C. 
Tth catalyzes the polymerization of nucleotides into duplex DNA in the 5´-->3´ direction in the presence of magnesium and the polymerization of nucleotides into DNA using an RNA template in the 5´-->3´ direction in the presence of manganese. The enzyme has a molecular weight of 94 000 daltons as estimated from the predicted amino acid sequence and exhibits 5´-->3´ exonuclease activity. Tth is recommended for use in PCR, RT-PCR, reverse transcription and primer extension reactions at elevated temperature.

Name	Quantity	Reference	EUR
Tth DNA polymerase	100 U	ME-0070-01	40.20
Tth DNA polymerase	500 U	ME-0070-05	160.50

Delivery times

   2 working days

Shipping conditions

   -20 °C

Storage conditions

   -20 °C

Description

Tth DNA Polymerase is a thermostable DNA polymerase derived from the thermophilic bacterium Thermus thermophilus. It has optimal synthetic activity at temperatures above 70 °C and can be used at temperatures up to 95 °C, making it useful for applications such as PCR. The enzyme also has thermostable reverse transcriptase activity, which enables synthesis of cDNA from RNA templates. The high reaction temperatures possible with Tth DNA Polymerase minimize problems associated with nonspecific priming and template secondary structure.

Source

  Thermus thermophilus

Applications

Tth polymerase is a highly thermostable enzyme especially recommended for the amplification of DNA fragments longer than 4 kb. Tth DNA polymerase also has a reverse transcriptase activity, making it suitable for RT-PCR.

Concentration

  5 U/µl

TDS

• Tth DNA polymerase

 

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