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qPCR MasterMix Plus

Research Diagnostic Therapeutic
qPCR MasterMix Plus Order
The qPCR MasterMix Plus contains enough reagents for up to 300 / 600 / 2000 - 50 µl reactions using the hotstart enzyme, HotGoldStar and has been validated on ABI prism® 5700, 7000, 7700, 7900, 7300, StepOne™ and MasterCycler® ep realplex.


NameQuantityReferenceEUR
qPCR MasterMix Plus 7.5 ml300RT-QP2X-03-075+469.00
qPCR MasterMix Plus 15 ml600RT-QP2X-03-15+929.00
qPCR MasterMix Plus 50 ml2000RT-QP2X-03-50+2795.00
50 µl reactions

Sample request

Delivery times

   2 WD if ordered before Thursday

Shipping conditions

   Dry ice

Storage conditions


For long term storage the qPCR MasterMix Plus should be stored at -15 °C to -25 °C in a constant temperature freezer. When stored under these conditions the reagents are stable for 2 years.
For short term storage the qPCR MasterMix Plus can be stored at 4 °C to 6 °C for 1 month.

Our qPCR MasterMix Plus will allow you to achieve reliable, consistent and accurate Real-Time qPCR results for probe detection. The qPCR MasterMix Plus contains enough reagents for up to 300 / 600 / 2000 - 50 µl reactions using the hotstart enzyme, HotGoldStar and has been validated on ABI prism® 5700, 7000, 7700, 7900, 7300, MasterCycler® ep realplex. The 2x buffer contains dNTP/dUTP, HotGoldStar, UNG, MgCl2, stabilizers and ROX passive reference. An extra tube of 50 mM MgCl2 is provided in the kit to optimize MgCl2 concentration if necessary.


Comparison between qPCR MasterMix Plus and competitor A reagents. Experiment was performed using same 18S ribosomal RNA primers and probe using same cDNA, same plate on an ABI Prism 7700.
Eurogentec reagents showed maximal efficiency, better sensitivity and higher RN compared to competitor A reagents.




Legal notices


FOR RESEARCH USE ONLY
NOTICE TO PURCHASER: LIMITED LICENSE
A license under U.S. Patents 4,683,202, 4,683,195 and 4,965,188 or their foreign counterparts, owned by Roche Molecular Systems, Inc. and F. Hoffmann-La Roche Ltd (Roche”), has an up-front fee component and a running-royalty component. The purchase price of this product includes limited, non-transferrable rights under the running-royalty component to use only this amount of the product to practice the Polymerase Chain Reaction (“PCR”) and related processes described in said patents solely for the research and development activities of the purchaser when this product is used in conjunction with a thermal cycler whose use is covered by the up-front fee component. Rights to the up-front fee component must be obtained by the end user in order to have a complete license. These rights under the up-front fee component may be purchased from Applied Biosystems or obtained by purchasing an Authorized Thermal Cycler. No right to perform or offer commercial services of any kind using PCR, including without limitation reporting the results of purchaser’s activities for a fee or other commercial consideration, is hereby granted by implication or estoppel. Further information on purchasing licenses to practice the PCR Process may be obtained by contacting the Director of Licensing at Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404 or the Licensing Department at Roche Molecular Systems, Inc., 1145 Atlantic Avenue, Alameda, California 94501.

The use of certain fluorogenic probes in 5' nuclease assays may be covered by U.S. Patents 5,210,015 and 5,487,972, owned by Roche Molecular Systems, Inc, and by U.S. Patent 5,538,848, owned by The Perkin-Elmer Corporation. The purchase of Eurogentec’s product does not provide a license to use this patented technology. A license must be obtained by contacting the Director of Licensing Applied Biosystems, 850 Lincoln Centre Drive, Foster City, CA 94404 or the Licensing Department at Roche Molecular Systems Inc., 1145 Atlantic Avenue, Almeda, CA 94501.

Use of UDG employs U.S. Patents 5,035,996, 5,945,313, 5,683,896 and their foreign counterparts licensed to Eurogentec, S.A. from Invitrogen Corporation.

Manual

Leaflets

TDS

MSDS

Certificate of analysis


To obtain the Certificate of Analysis corresponding to your qPCR kit of interest please contact us via email and display the reference code of the qPCR kit and the batch number. Contact us

Product citations


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YANG Z. et al., "Protein Kinase B /Akt1 Regulates Placental Development and Fetal Growth", Journal of Biological Chemistry, vol.278, n° 34, p. 32124-32131, May 2003


WANG S. et al., "Histone deacetylase 1 represses the small GTPase RhoB expression in human nonsmall lung carcinoma cell line", Oncogene, n° 22, p. 6204-6213, 2003


STIEWE T. et al., "Quantitative TP73 Transcript Analysis in Hepatocellular Carcinomas", Clinical Cancer Research, Vol. 10, 626-633, January 2004


SWILLENS S. et al., "Instant evaluation of the absolute initial number of cDNA copies from a single real-time PCR curve", Nucleic Acids Research, vol. 32, n° 6, March 2004


LÖFSTEDT T. et al., "Induction of ID2 Expression by Hypoxia-inducible Factor-1", Journal of Biological Chemistry, vol. 279, n° 38, 39223-39231, September 2004


TAKEI K. et al., "Arabidopsis CYP735A1 and CYP735A2 Encode Cytokinin Hydroxylases That Catalyze the Biosynthesis of trans-Zeatin", Journal of Biological Chemistry, vol. 279, n° 40, p. 41866-41872, October 2004


FOURMESTRAUX V. et al., "Transcript Profiling Suggests That Differential Metabolic Adaptation of Mice to a High Fat Diet Is Associated with Changes in Liver to Muscle Lipid Fluxes", Journal of Biological Chemistry, vol. 279, n° 49, 50743-50753, December 2004


PIERREUX Chr. et al., "The Transcription Factor Hepatocyte Nuclear Factor-6/Onecut-1 Controls the Expression of Its Paralog Onecut-3 in Developing Mouse Endoderm", Journal of Biological Chemistry, vol. 279, n° 49, 51298-51304, December 2004


ROEBROEK A. et al., "Limited Redundancy of the Proprotein Convertase Furin in Mouse Liver", Journal of Biological Chemistry, vol. 279, n° 51, 53442-53450, December 2004


BAETZ A. et al., "Suppressor of Cytokine Signaling (SOCS) Proteins Indirectly Regulate Toll-like Receptor Signaling in Innate Immune Cells", Journal of Biological Chemistry, vol. 279, n° 52, 54708-54715, December 2004


FERRARI S. et al., "Chromatin Domain Boundaries Delimited by a Histone-binding Protein in Yeast", Journal of Biological Chemistry, vol. 279, n° 53, 55520-55530, December 2004


VAN LEEUWEN D. M. et al., "Differential Gene Expression in Human Peripheral Blood Mononuclear Cells Induced by Cigarette Smoke and Its Constituents", Toxicological Sciences, 86(1), 200-210, April 2005


DEKAIRELLE A.-F. et al., "Assessment of the Transcriptional Activity of p53 Improves the Prediction of Recurrence in Superficial Transitional Cell Carcinoma of the Bladder", Clinical Cancer Research, vol. 11, 4724-4732, July 1, 2005


EBNER K. et al., "Molecular Detection and Quantitative Analysis of the Entire Spectrum of Human Adenoviruses by a Two-Reaction Real-Time PCR Assay", Journal of Clinical Microbiology, vol. 43, n° 7, p. 3049-3053, July 2005


Ishibashi Y. et al., "Regulation of sialyl-Lewis x epitope expression by TNF-alpha and EGF in an airway carcinoma cell line", Glycoconjugate Journal, 22(1-2):53-62, February 2005


KUROSAWA Y. et al., "Novel method to examine hepatocyte-specific gene expression in a functional coculture system", Tissue Engineering, 11(11-12):1650-7, Nov-Dec 2005


GLASS J. et al., "Essential genes of a minimal bacterium", PNAS, vol. 103, no. 2, 425-430, January 10, 2006


SCHOLZ B. et al., "Sex-dependent gene expression in early brain development of chicken embryos", BMC Neuroscience, 15;7:12, February 2006


DECOUSSER J.-W. et al., "New Real-Time PCR Assay Using Locked Nucleic Acid Probes To Assess Prevalence of ParC Mutations in Fluoroquinolone-Susceptible Streptococcus pneumoniae Isolates from France", Antimicrobial Agents and Chemotherapy, p. 1594-1598, Vol. 50, No. 4, April 2006




Troubleshooting guide

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  • By fax : +32 4 264 07 88
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    Eurogentec S.A.
    LIEGE Science Park
    Rue du Bois Saint Jean 5
    4102 Seraing
    BELGIUM

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