Recombinant rat MOG Protein

Life Science Molecular Diagnostics GMP BioManufacturing

The sequence (Accession #CAE84068) corresponding to the extracellular domain of rat MOG along with a 6x His tag was expressed in E. coli. The recombinant rat MOG (R-rMOG) was purified from urea denatured bacterial lysate using immobilized metal affinity chromatography (IMAC). The molecular weight of the recombinant rat MOG is 14.2 kDa.

NamePack sizeReference
Recombinant Rat MOG Protein100 µgAS-55152-100
Recombinant Rat MOG Protein500 µgAS-55152-500
Recombinant Rat MOG Protein1000 µgAS-55152-1000


Host

   Rat


Activity

Female DA rats and Lewis rats (10 weeks old) were immunized (tail base s.c.) with 50 μg/animal of rat rMOG in complete Freund’s adjuvant. DA and Lewis rats showed EAE symptoms such as limp tail, hind limb weakness, hind limb paralysis, and weight loss after induction. Please note that no other EAE induction protocols were tested including IFA/cytokine model.

Delivery times

   10 working days

Shipping conditions

   Dry Ice

Storage conditions


The purified rat rMOG is supplied as sterile and frozen at 1 mg/ml in 20 mM sodium acetate buffer (pH=4.0). Store at 2-4 ºC for immediate use within 3 weeks or at -80 ºC for up to 12 months. Avoid repeated freeze-thaw cycles.
Myelin Oligodendrocyte Glycoprotein (MOG) is a member of the immunoglobulin superfamily and is expressed exclusively in central nervous system (CNS). Although MOG protein constitutes only 0.01-0.05% of the CNS myelin proteins, it was demonstrated that MOG protein is a crucial autoantigen for multiple sclerosis in humans and experimental autoimmune encephalomyelitis (EAE) in rodents and monkeys (1-5). The purified mouse rMOG is recommended for in vitro studies such as T cell and B cell responses, cytokine response, antigen presentation, Western blotting, and ELISA as well as for in vivo study such as EAE induction in mice. The following dosages are recommended: 5-20 μg/ml for in vitro study and 50 μg per animal for in vivo study (1-5).

References

  1. Jayaram Bettadapura et.al. (1998) Journal of Neurochemistry 70 (4): 1593-1599
  2. Alfred R Oliver et al (2003) Journal of Immunology 171:462-468
  3. Hans-Christian Von Budingen et.al. (2001) Journal of Clinical Immunology 21 (3): 155-170
  4. Jerri-Anne Lyons et.al. (1999) European Journal of Immunology 29: 3432-3439
  5. Hans-Christian Von Budingen et.al. (2004) European Journal of Immunology 34: 2072-2083

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  • Online ordering: The most convenient way to order your oligonucleotides, peptides and Real-Time qPCR kits is to use our web ordering system. To place orders online, simply login if you have already an online account.

    New online customers ? Not registered yet ? Request your free login and password.

  • By e-mail: order@eurogentec.com
  • By fax : +32 4 264 07 88
  • By mail
    Eurogentec S.A.
    LIEGE Science Park
    Rue du Bois Saint Jean 5
    4102 Seraing
    BELGIUM

If you still have questions with ordering please contact our Customer Support : +32 4 372 76 65.

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