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Research Diagnostic Therapeutic

Service package includes S. pneumoniae MicroArray, two RNA labelling reactions, dual hybridization, slide scanning, image extraction and data analysis

Name	Quantity	Reference	EUR
S. Pneumoniae Hybridisation services	1	AR-HYSP-01	1090.00

Quality Control

100 % satisfaction guaranteed

Delivery times

10 WD

Hybridization services and data analysis

1- Package Service package includes, custom MicroArrays or our catologue MicroArrays, RNA labeling, hybridization, slide scanning, image extraction and data analysis

2- Receiving samples The RNA samples should be sent in dry ice at the minimal concentration of 2 micrograms/microliter in DEPC treated water, to Eurogentec Microarray Department, during regular business hours, Monday to Friday, 9:00 am - 5:00 pm. The RNA samples can be sent by express mail service. For gene expression analysis, we required 25 micrograms of total RNA per sample.

3- RNA quality control You will need to extract total RNA, provide a photo of a gel and the 260/280 optical density ratio to assure minimum RNA quality. Eurogentec's Microarray Department will run RNA quality control procedure using Bioanalyzer 2100 instrument to ensure RNA quality before Microarray experiment. If the quality is not meeting the requirement, you will be noticed and asked to ship new RNA preparation.

4-RNA labeling We use the use direct labeling methods, during cDNA synthesis the CY3-dCTP and CY5-dCTP is incorporate to first strand cDNA.

5-Hybridization: The total labeling cDNA is applied on C. albicnas MicroArray for hybridization on Array Booster(tm) station, which guarantee fast and reproducible hybridization results. The slide are washed at stringent conditions and scanned immediately on GenePix 4000B scanner, which is able to scan two wavelength excitations at 532 (Cy3) and 635 (Cy5) nm in a single phase.

6- The Microarray results including TIFF And JPEG images of hybridization and data analysis Excel(tm) file will be recorded onto CD and sent to you by express mail.

7- Service turn around time The Microarray hybridization results will be returned to user within 10 working days upon reception of RNA samples and the official purchasing order.