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siRNA control kits

Research Diagnostic Therapeutic
Eurogentec is introducing a range of complete validation kits with validated sequences and reagents which enable you to control your experiment conditions at both mRNA level and protein level.


Name Reference EUR
Cyclophilin OR-KIT1-Cyclophilin 545.00
Luciferase OR-KIT1-LUC 545.00
Beta-actin OR-KIT1-BACTIN 545.00
GADPH OR-KIT1-GADPH 545.00


Storage conditions

   -20 °C to -70 °C
Store siRNA oligos as a dry pellet at -20°C (or preferably -70°C) in a non-frost free freezer until ready to use. Once resuspended in RNase-free buffer, store at -70°C and avoid contact with RNases. siRNA oligos should be resuspended to a convenient stock concentration (20 to 50 µM) and stored in small aliquots to avoid multiple freeze thaw cycles. When stored under these conditions and using good RNase-free technique, they typically remain stable for 6 months or more. The solution can be freeze-thawed up to 5 times. For long-term storage, siRNA oligos should be dried.

Eurogentec is introducing a range of complete validation kits with validated sequences and reagents which enable you to control your experiment conditions at both mRNA level and protein level.

Kit content

  • 1 siRNA control duplex
  • 1 Double-Dye probe
  • 2 OliGold® PCR primers validated for probe assay
  • 1 antibody

Quantities per kit:

  • siRNA: 5 nmol
  • Double-Dye probe: 1.5 nmol
  • Primers pair: 5 nmol of each primer
  • Antibody: variable, sufficient quantity to perform 25 Western Blot experiments.

We are continuously updating this list of validated sequences. If the one that you need is not included, please inquire.


Result validation

In order to validate the result of your RNAi experiment, it is highly recommended to measure the gene silencing efficiency. This validation can be made at two levels; either at the mRNA level using the Real-Time qPCR or Northern Blot techniques, or at the protein level with specific antibodies against the target protein (e.g. Western Blotting). Thanks to the tools that Eurogentec proposes, you can choose to validate your experiment at one or two levels.


Tools

  • Real-Time qPCR probe
    Eurogentec is very active in the Real-Time qPCR market. Every reagents and probes types are available and produced under the highest quality criteria. To validate your siRNA experiment ask us to provide you with a designed probe for evaluating the level of the mRNA in your cells.
  • Antibodies
    The Proteomics Unit of Eurogentec is providing customized polyclonal and monoclonal antibodies and antibodies from the shelf. A full range of services are also provided from the design of the epitope to the delivery of the antibody.

Legal notices

   For Research Use only

Product citations


POTENTE M. et al., "11,12-Epoxyeicosatrienoic Acid-induced Inhibition of FOXO Factors Promotes Endothelial Proliferation by Down-Regulating p27Kip1", Journal of Biological Chemistry, vol. 278, n° 32, p. 29619-29625, May 2003


TUROWSKI P. et al., "Functional cdc25C Dual-Specificity Phosphatase Is Required for S-Phase Entry in Human Cells", Molecular Biology of the Cell, vol. 14, Issue 7, p. 2984-2998, July 2003


AVEROUST J. et al., "Induction of CHOP Expression by Amino Acid Limitation Requires Both ATF4 Expression ATF2 Phosphorylation", Journal of Biological Chemistry, vol. 279, n° 7, p. 5288-5297, February 2004


LAZRAK M. et al., "The bHLH TAL-1/SCL regulates endothelial cell migration and morphogenesis", Journal of Cell Science, 117, 1161-1171, February 2004


DUTTON A. et al., "Expression of the cellular FLICE-inhibitory protein (c-FLIP) protects Hodgkin's lymphoma cells from autonomous Fas-mediated death", PNAS, vol. 101, n° 17, p. 6611-6616, April 2004


VOURET-CRAVIARI V. et al., "ILK is required for the assembly of matrix-forming adhesions and capillary morphogenesis in endothelial cells", Journal of Cell Science, 117, p. 4559-4569, May 2004


YU L.-G. et al., "Protein Phosphatase 2A, a Negative Regulator of the ERK Signaling Pathway, Is Activated by Tyrosine Phosphorylation of Putative HLA Class II-associated Protein I (PHAPI)/pp32 in Response to the Antiproliferative Lectin, Jacalin", Journal of Biological Chemistry, vol. 279, n° 40, p. 41377-41383, October 2004


STEWART S. et al., "Epstein–Barr virus-encoded LMP2A regulates viral and cellular gene expression by modulation of the NF-B transcription factor pathway", PNAS, vol. 101, n° 44, 15730-15735, November 2004


LAIGLE A. et al., "The reduction of P-Glycoprotein Expression by small interfering RNAs is improved in exponentially growing cells", Oligonucleotides, 14:191-198, 2004


WÜNSCHE W. et al., "The activity of siRNA in Mammalian Cells is related to the kinetics of siRNA-target recognition in vitro: Mechanistic Implications", Journal of Molecular Biology, vol. 345, 203-209, 2005


LOUIS K. et al., "Tumor Cell-mediated Induction of the Stromal Factor Stromelysin-3 Requires Heterotypic Cell Contact-dependent Activation of Specific Protein Kinase C Isoforms", Journal of Biological Chemistry, vol. 280, n° 2, p. 1272-1283, January 2005





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    You can place your order online. Login to access our web ordering interface.
  • By fax : +32 4 264 07 88
  • By mail
    Eurogentec S.A.
    LIEGE Science Park
    Rue du Bois Saint Jean 5
    4102 Seraing
    BELGIUM

If you still have questions with ordering please contact our Customer Support : +32 4 372 76 65.

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