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Smart kit Order

The Smart kit contains enough reagents for up to 500 - 50 µl reactions using the hotstart enzyme, HotGoldStar and has been validated on Smartcyler® thermocyclers.

Name	Quantity	Reference
Smart kit	500	RT-QP2X-CE

25 µl reactions

Delivery times

2 WD if ordered before Thursday

Shipping conditions

Dry ice

Storage conditions

For long term storage the Smart kit should be stored at -15 °C to -25 °C in a constant temperature freezer. When stored under these conditions the reagents are stable for 2 years.
For short term storage the Smart kit can be stored at 4 °C to 6 °C for 1 month.

Our Smart kit will allow you to achieve reliable, consistent and accurate Real-Time qPCR results for probe detection. The Smart kit contains enough reagents for up to 500 - 50 µl reactions using the hotstart enzyme, HotGoldStar and has been validated onSmartcycler® thermocyclers. The 2x buffer contains dNTP/dUTP, HotGoldStar, UNG, MgCl2 and stabilizers. An extra tube of 50 mM MgCl2 is provided in the kit to optimize MgCl2 concentration if necessary.

Legal notices

FOR RESEARCH USE ONLY
NOTICE TO PURCHASER: LIMITED LICENSE
A license under U.S. Patents 4,683,202, 4,683,195 and 4,965,188 or their foreign counterparts, owned by Roche Molecular Systems, Inc. and F. Hoffmann-La Roche Ltd (Roche”), has an up-front fee component and a running-royalty component. The purchase price of this product includes limited, non-transferrable rights under the running-royalty component to use only this amount of the product to practice the Polymerase Chain Reaction (“PCR”) and related processes described in said patents solely for the research and development activities of the purchaser when this product is used in conjunction with a thermal cycler whose use is covered by the up-front fee component. Rights to the up-front fee component must be obtained by the end user in order to have a complete license. These rights under the up-front fee component may be purchased from Applied Biosystems or obtained by purchasing an Authorized Thermal Cycler. No right to perform or offer commercial services of any kind using PCR, including without limitation reporting the results of purchaser’s activities for a fee or other commercial consideration, is hereby granted by implication or estoppel. Further information on purchasing licenses to practice the PCR Process may be obtained by contacting the Director of Licensing at Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404 or the Licensing Department at Roche Molecular Systems, Inc., 1145 Atlantic Avenue, Alameda, California 94501.

The use of certain fluorogenic probes in 5' nuclease assays may be covered by U.S. Patents 5,210,015 and 5,487,972, owned by Roche Molecular Systems, Inc, and by U.S. Patent 5,538,848, owned by The Perkin-Elmer Corporation. The purchase of Eurogentec’s product does not provide a license to use this patented technology. A license must be obtained by contacting the Director of Licensing Applied Biosystems, 850 Lincoln Centre Drive, Foster City, CA 94404 or the Licensing Department at Roche Molecular Systems Inc., 1145 Atlantic Avenue, Almeda, CA 94501.

Use of UDG employs U.S. Patents 5,035,996, 5,945,313, 5,683,896 and their foreign counterparts licensed to Eurogentec, S.A. from Invitrogen Corporation.

Leaflets

TDS

Download the Smart Kit Technical Data Sheet now

MSDS

Certificate of analysis

To obtain the Certificate of Analysis corresponding to your qPCR kit of interest please contact us via email and display the reference code of the qPCR kit and the batch number. Contact us

Product citations

GOTTAR M. et al., The Drosophila immune response against Gram-negative bacteria is mediated by a peptidoglycan recognition protein", Nature, n° 416, p. 640-644, 11 April 2002

LAURITZEN I. et al., "K⁺ -dependent Cerebellar Granule Neuron Apoptosis", Journal of Biological Chemistry, vol.278, n° 34, p. 32068-32076, May 2003

YANG Z. et al., "Protein Kinase B /Akt1 Regulates Placental Development and Fetal Growth", Journal of Biological Chemistry, vol.278, n° 34, p. 32124-32131, May 2003

WANG S. et al., "Histone deacetylase 1 represses the small GTPase RhoB expression in human nonsmall lung carcinoma cell line", Oncogene, n° 22, p. 6204-6213, 2003

STIEWE T. et al., "Quantitative TP73 Transcript Analysis in Hepatocellular Carcinomas", Clinical Cancer Research, Vol. 10, 626-633, January 2004

SWILLENS S. et al., "Instant evaluation of the absolute initial number of cDNA copies from a single real-time PCR curve", Nucleic Acids Research, vol. 32, n° 6, March 2004

LÖFSTEDT T. et al., "Induction of ID2 Expression by Hypoxia-inducible Factor-1", Journal of Biological Chemistry, vol. 279, n° 38, 39223-39231, September 2004

TAKEI K. et al., "Arabidopsis CYP735A1 and CYP735A2 Encode Cytokinin Hydroxylases That Catalyze the Biosynthesis of trans-Zeatin", Journal of Biological Chemistry, vol. 279, n° 40, p. 41866-41872, October 2004

FOURMESTRAUX V. et al., "Transcript Profiling Suggests That Differential Metabolic Adaptation of Mice to a High Fat Diet Is Associated with Changes in Liver to Muscle Lipid Fluxes", Journal of Biological Chemistry, vol. 279, n° 49, 50743-50753, December 2004

PIERREUX Chr. et al., "The Transcription Factor Hepatocyte Nuclear Factor-6/Onecut-1 Controls the Expression of Its Paralog Onecut-3 in Developing Mouse Endoderm", Journal of Biological Chemistry, vol. 279, n° 49, 51298-51304, December 2004

ROEBROEK A. et al., "Limited Redundancy of the Proprotein Convertase Furin in Mouse Liver", Journal of Biological Chemistry, vol. 279, n° 51, 53442-53450, December 2004

BAETZ A. et al., "Suppressor of Cytokine Signaling (SOCS) Proteins Indirectly Regulate Toll-like Receptor Signaling in Innate Immune Cells", Journal of Biological Chemistry, vol. 279, n° 52, 54708-54715, December 2004

FERRARI S. et al., "Chromatin Domain Boundaries Delimited by a Histone-binding Protein in Yeast", Journal of Biological Chemistry, vol. 279, n° 53, 55520-55530, December 2004

VAN LEEUWEN D. M. et al., "Differential Gene Expression in Human Peripheral Blood Mononuclear Cells Induced by Cigarette Smoke and Its Constituents", Toxicological Sciences, 86(1), 200-210, April 2005

DEKAIRELLE A.-F. et al., "Assessment of the Transcriptional Activity of p53 Improves the Prediction of Recurrence in Superficial Transitional Cell Carcinoma of the Bladder", Clinical Cancer Research, vol. 11, 4724-4732, July 1, 2005

EBNER K. et al., "Molecular Detection and Quantitative Analysis of the Entire Spectrum of Human Adenoviruses by a Two-Reaction Real-Time PCR Assay", Journal of Clinical Microbiology, vol. 43, n° 7, p. 3049-3053, July 2005

Ishibashi Y. et al., "Regulation of sialyl-Lewis x epitope expression by TNF-alpha and EGF in an airway carcinoma cell line", Glycoconjugate Journal, 22(1-2):53-62, February 2005

KUROSAWA Y. et al., "Novel method to examine hepatocyte-specific gene expression in a functional coculture system", Tissue Engineering, 11(11-12):1650-7, Nov-Dec 2005

GLASS J. et al., "Essential genes of a minimal bacterium", PNAS, vol. 103, no. 2, 425-430, January 10, 2006

SCHOLZ B. et al., "Sex-dependent gene expression in early brain development of chicken embryos", BMC Neuroscience, 15;7:12, February 2006

DECOUSSER J.-W. et al., "New Real-Time PCR Assay Using Locked Nucleic Acid Probes To Assess Prevalence of ParC Mutations in Fluoroquinolone-Susceptible Streptococcus pneumoniae Isolates from France", Antimicrobial Agents and Chemotherapy, p. 1594-1598, Vol. 50, No. 4, April 2006

Troubleshooting guide

Download the TroubleShooting Guide now

How to order by e-mail

Send your orders by email. To get the template ordering form, please contact our Customer Help Desk or send an e-mail to info@eurogentec.com.
Do not hesitate to contact us to set up the best ordering method for you.

How to order by fax

It is possible to order your product by fax; please check the fax numbers at the bottom of each page of our catalogue or below. This ordering method requires more manpower than the previously mentioned ones any specifications in our system. Moreover we are applying a double check to avoid any mistakes. The treatment of these orders requires one additional working day to the standard delivery times. For fax orders an additional fee of 2.50 eur per product will be charged. However, we do not recommend fax ordering, because these orders have to be processed manually and are therefore errorprone.

How to order by mail

It is possible to order your Real Time PCR reagents and miscelleneous by mail; please use the following adress:

Do not hesitate to contact us for any additional information or comments on these ordering tools.

How to order online

Order your Real Time qPCR reagents on our secured website. Click on “how to order” and follow the instructions, you will receive your personal Login and Password within a few minutes. Using this ordering page, you will have access to every product in our Real Time qPCR range : Real Time qPCR reagents, plastics, control kits.