The SolulinK Conjugation System!
Finally, a conjugation chemistry that is simple to apply, stable in solution, and applicable to almost any conjugation project ! The Solulink chemistry is highly selective, stable in solution and not susceptible to non-specific binding, making it superior to conventional methods of conjugation. The advantages include its reaction specificity, UV-traceability, and the unique control it brings to the entire conjugation process. Specifically, the method readily reacts with primary amines on a protein (ε-amino group of lysine) via an NHS-ester. There is also a built-in traceable HyNic group (hydrazinonicotinate) in the conjugated proteins or other biomolecules that provides a confirmation and quantification of the conjugation. HyNic-modified proteins then react to form stable conjugates in the presence of other (aromatic) aldehyde-modified proteins or biomolecules.
What makes Solulink Linker Chemistry Different?
Traceability- The bis-aryl hydrazone bond formed with HyNic absorbs at 354 nm with a molar extinction coefficient of 29,000.
Reproducibility- Biomolecule modification can be quantified for greater batch-to-batch reproducibility.
Linker stability- Biomolecules modified with hydrazines and aldehydes have extended stabilities.
Specific- Solulink Bioconjugation chemistry assures formation of heteroconjugates, with no formation of homoconjugates. Reactive moieties on modified biomolecules are inert and do not react with protein functional groups.
Versatility- With our linkers it is simple to conjugate any biomolecule to another or to solid surfaces like beads or arrays. Example conjugates include: antibody–oligo, antibody-enzyme, antibody-fluor, peptide-fluor, peptide-KLH and many others.
ChromaLinK™ Biotin, Biotinylate & Quantify with Just One Reagent! NO HABA Assay needed.
Application: ChromaLinkTM Biotin incorporates biotin on amine containing biomolecules or surfaces and allows for direct spectrophotometric quantitation of total biotin incorporation on any biomolecule.
Technology: CLB354S has been designed to (a) incorporate a bis-aryl hydrazone chromophore (29,000 @ 354 nm) in the chain that allows for direct spectroscopic quantitation of total incorporated biotins, a long chain PEG4 linker to preserve biotin/avidin affinity as well as increase solubility and an aromatic succinimidyl ester that more efficiently modifies amines in aqueous buffers than aliphatic succinimidyl esters.
By measuring the A280 and A354 of the modified biomolecule the protein concentration and number of biotins incorporated/protein can be determined directly. As little as 20 µg aliquot of modified protein in 100 µl buffer in a microplate assay will yield reproducible results.
To know more about Solulink products, download Solulink 2008 catalogue!