This consists of a bi-labelled probe with a fluorescent dye at the 5’ end (FAM, HEX, TET, JOE, Yakima Yellow®, ROX, ...) and an internal non-fluorescent quencher.
| Name |
Synthesis scale |
Recommended Purification* |
Included Purification* |
Reference |
EUR |
|
| Standard Scorpions® primers |
200 nmol |
N/A |
N/A |
PB-SP001-020 |
535.50 |
|
* More info on the Purifications page Quality Control MALDI-TOF Mass Spectrometry and Analytical HPLC Delivery times 15-39 bases: 12 Working days 40-80 bases: 15 Working days Packaging Lyophilised Shipping conditions Room temperature Storage conditions -20 °C to -70 °C Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Lyophilized DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.
This consists of a bi-labelled probe with a fluorescent dye at the 5’ end (FAM, HEX, TET, JOE, Yakima Yellow®, ROX, ...) and an internal non-fluorescent quencher. Scorpions® Advantages : - Stronger signals and lower background
Typical Real-Time qPCR profiles obtained with Scorpions® primers demonstrate the low backgrounds and high signals of this type of probe in contrast to the high background of Double-Dye Oligonucleotides, and the low signal intensities associated with Molecular Beacons. - Support for rapid cycling
Because the Scorpions® reactions occur effectively instantaneously, there is no need for an extended extension phase to allow signal generation to take place. - Improved SNP testing
Scorpions® can be used for SNP analysis in two formats: allele specific hybridization or, uniquely, allele specific extension (ARMs technology). Allele specific Scorpions® primers give improved discrimination over other systems due to their unimolecular mode of action, and the possibility of an alternative structure that the probe element can form in preference to hybridizing to the mismatched target. - Extended multiplexing
Scorpions® are compatible with any fluorescent dye, and this coupled with the low backgrounds of Scorpions® reactions allows extended multiplex development. Using a Real-Time PCR device with a broad range of filter, it is possible to combine four or more separate reactions. Eurogentec is a licensed supplier of Scorpions® and offers a wide range of fluorophore reporters and quenchers. Scorpions® quenched with Deep Dark Quencher I, and coupled with a variety of fluorescent reporters can be conveniently ordered using our new web ordering interface. Scorpions® are double-HPLC purified and controlled by MALDI-TOF Mass Spectometry. The maximum length of Scorpions® probes is 45 bases. Scorpions® probes are provided lyophilized in individual tubes.
Eurogentec determines the signal-to-noise ratio (SNTR) for each Scorpions® by hybridizing it to its complementary sequence and comparing fluorescence to background. For FAM labelled Scorpions®, guaranteed SNTR is > 15. All other dyes require a signal-to-noise ratio of > 10 to pass Quality Control.
Legal notices For Research Use only
Leaflets Product citations THELWELL N. et al., "Mode of action and application of Scorpion primers to mutation detection", Nucleic Acids Research, vol. 28, n° 19, p. 3752-3761, October 2000 HART K.W. et al., "Novel method for detection, typing, and quantification of human papillomaviruses in clinical samples", Journal of Clinical Microbiology, vol. 39, n° 9, p. 3204-3212, September 2001 SOLINAS A. et al., "Duplex Scorpion primers in SNP analysis and FRET applications", Nucleic Acids Research, vol. 29, n° 20, October 2001 SCHENA L. et al., "Identification and Detection of Rosellinia Necatrix by Conventional and Real-time Scorpion-PCR", European Journal of Plant Pathology, vol. 108, n° 4, p. 355-366(12), May 2002 SCHENA L. et al., "Molecular Detection of Strain L47 of Aureobasidium pullulans, a Biocontrol Agent of Postharvest Diseases", Plant Disease, vol. 86, n° 1, 54-60, 2002 SCHENA L. and IPPOLITO A., "Rapid and sensitive detection of Rosellinia necatrix in roots and soils by Real-Time Scorpions-PCR", Journal of Plant Pathology, 85 (1), 15-25, January 2003 IPPOLITO A. et al., "Real-Time detection of Phytophthora nicotianae and P. citrophthora in citrus roots and soil", European Journal of Plant Pathology, vol. 110, 833-843, 2004 SCHENA L. et al., "Real-Time quantitative PCR: a new technology to detect and study phytopathogenic and antagonistic fungi", European Journal of Plant Pathology, vol. 110, 893-908, 2004
- Online ordering: The most convenient way to order your oligonucleotides, peptides and Real-Time qPCR kits is to use our web ordering system. To place orders online, simply login if you have already an online account.
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- By e-mail: order@eurogentec.com
- By mail
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4102 Seraing
BELGIUM
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