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Thiol modifiers Build & Order

Thiol (SH) group can be linked to the 5’ or 3’ terminus of an oligonucleotide. This modification is used for oligo activation prior to linking to various types of molecules or support. The thiol groups can be used to attach a variety of products to the oligonucleotide. Common thiol-reactive groups include maleimides, halogens, iodoacetamides and pyridyldisulfides. This DNA modification is commonly used for the attachment of enzymes such as horseradish peroxidase or alkaline phosphatase.

Name	Synthesis scale	Recommended Purification*	Required Purification*	Reference	EUR
5' Thiol Modifier C6	40 nmol	RP-HPLC	-	MD-MF056-05004	36.00
5' Thiol Modifier C6	200 nmol	RP-HPLC	-	MD-MF056-05020	48.00
5' Thiol Modifier C6	1000 nmol	RP-HPLC	-	MD-MF056-05100	63.00
5' Thiol Modifier C6	2.5 µmol	RP-HPLC	-	MD-MF056-05M02	245.80
5' Thiol Modifier C6	5 µmol	RP-HPLC	-	MD-MF056-05M05	399.50
5' Thiol Modifier C6	10 µmol	RP-HPLC	-	MD-MF056-05M10	770.10
3' Thiol Modifier C3	40 nmol	RP-HPLC	-	MD-MF053-03004	57.80
3' Thiol Modifier C3	200 nmol	RP-HPLC	-	MD-MF053-03020	84.50
3' Thiol Modifier C3	1000 nmol	RP-HPLC	-	MD-MF053-03100	130.70
3' Thiol Modifier C6	40 nmol	RP-HPLC	-	MD-MF056-03004	57.80
3' Thiol Modifier C6	200 nmol	RP-HPLC	-	MD-MF056-03020	84.50
3' Thiol Modifier C6	1000 nmol	RP-HPLC	-	MD-MF056-03100	130.70
3' Thiol Modifier C6	2.5 µmol	RP-HPLC	-	MD-MF056-03M02	245.80
3' Thiol Modifier C6	5 µmol	RP-HPLC	-	MD-MF056-03M05	399.50
3' Thiol Modifier C6	10 µmol	RP-HPLC	-	MD-MF056-03M10	770.10
3' Thiophosphate	40 nmol	RP-HPLC	-	MD-MF060-03004	47.60
3' Thiophosphate	200 nmol	RP-HPLC	-	MD-MF060-03020	58.00
3' Thiophosphate	1000 nmol	RP-HPLC	-	MD-MF060-03100	71.00
5' Thiophosphate	40 nmol	RP-HPLC	-	MD-MF060-05004	47.60
5' Thiophosphate	200 nmol	RP-HPLC	-	MD-MF060-05020	58.00
5' Thiophosphate	1000 nmol	RP-HPLC	-	MD-MF060-05100	71.00

* More info on the Purifications page

Quality Control

MALDI-TOF Mass Spectrometry

Delivery times

   2-14 bases: 5 Working days
                             15-39 bases: 5 Working days
                             40-80 bases: 7-8 Working days
                             > 80 bases: 10 Working days

Packaging

Dried

Shipping conditions

Room temperature

Storage conditions

-20 °C to -70 °C

Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.

Legal notices

For Research Use only

Order Forms

Oligo Order Form

Leaflets

Oligonucleotides Catalogue

Product citations

LEGENDRE D. et al., "Engineering a regulatable enzyme for homogeneous immunoassays", Nature Biotechnology, vol. 17, n° 1, p.67-72, 1 January 1999

MOMENI P. et al., "Mutations in a new gene, encoding a zinc-finger protein, cause tricho-rhino-phalangeal syndrome type I", Nature Genetics, vol. 24, p. 71-74, 1 January 2000

PEREL Y. et al., "Galanin and galanin receptor expression in neuroblastic tumours: correlation with their differentiation status", British Journal of Cancer, vol. 86, n° 1, p. 117-122, 7 January 2002

OSTERMANN G. et al., "JAM-1 is a ligand of the 2 integrin LFA-1 involved in transendothelial migration of leukocytes", Nature Immunology, vol. 3, n° 2, p.151-158, 1 February 2002

GOMEZ D. et al., "Interaction of Telomestatin with the Telomeric Single-strand Overhang", Journal of Biological Chemistry, vol. 279, n° 40, p. 41487-41494, October 2004

RZEM et al., "A gene encoding a putative FAD-dependent L-2-hydroxyglutarate dehydrogenase is mutated in L-2-hydroxyglutaric aciduria", PNAS, vol. 101, n° 48, 16849-16854, November 2004

SU T.-J. et al., "DNA bending by M.EcoKI methyltransferase is coupled to nucleotide flipping", Nucleic Acids Research, vol. 33, n° 10, 3235-3244, June 2005

VAN DER STEEGE G. et al., "Persistent failures in gene repair", Nature Biotechnology, n° 19, p. 305-306, April 2001

ISCHENKO A.A. et al., "Alternative nucleotide incision repair pathway for oxidative DNA damage", Nature, n° 415, p. 183-187, 10 January 2002

KRISTENSEN A. et al., "Detection of Mutations in Exon 8 of TP53 by Temperature Gradient 96-Capillary Array Electrophoresis", Biotechniques, n° 33, p. 650-653, September 2002

VANDEN ABEELE F. et al., "Store-operated Ca²⁺ Current in Prostate Cancer Epithelial Cells", Journal of Biological Chemistry, vol. 278, n° 17, p. 15381-15389, April 2003

GROS L. et al., "Hijacking of the Human Alkyl-N-purine-DNA Glycosylase by 3,N⁴-Ethenocytosine, a Lipid Peroxidation-induced DNA Adduct", Journal of Biological Chemistry, vol. 279, n° 17, p. 17723-17730, April 2004

MORGAN M. et al., "YY1 Regulates the Neural Crest-associated slug Gene in Xenopus laevis", Journal of Biological Chemistry, vol. 279, n° 45, p. 46826-46826, November 2004

DI GIUSTO D. et al., "Construction, Stability, and Activity of Multivalent Circular Anticoagulant Aptamers", Journal of Biological Chemistry, vol. 279, n° 45, p. 46483-46489, November 2004

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