The Two step RT qPCR MasterMix for SYBR® Green I No ROX contains enough reagents for up to 600- 25 µl RT and qPCR reactions using the hotstart enzyme, HotGoldStar and has been validated on Mx4000®, Mx3000P®, Mx3005P®, MasterCycler® ep realplex, iCycler iQ®, My iQ®, iQ5, DNA Engine Opticon® 1, DNA Engine Opticon® 2, Chromo 4, Mini Opticon®, Quantica®, LC480.
Fluorecein additive should be used for iCycler iQ®, My iQ®, iQ5.
| Name |
Quantity* |
Reference |
EUR |
|
| Two step qRT-PCR MasterMix for SYBR® Green I No ROX 7.5 ml |
600 |
RT-SN2X-03NRRT |
984.00 |
|
| 5 x Two step qRT-PCR MasterMix for SYBR® Green I No ROX 7.5 ml |
5 x 600 |
05-SN2X-03NRRT |
3690.00 |
|
| 10 x Two step qRT-PCR MasterMix for SYBR® Green I No ROX 7.5 ml |
10 x 600 |
10-SN2X-03NRRT |
5900.00 |
|
25 µl qPCR reactions 10 µl RT reactionsDelivery times 2 WD if ordered before Thursday Shipping conditions Dry ice Storage conditions
For long term storage the qPCR MasterMix Plus for SYBR® Green I No ROX should be stored at -15 °C to -25 °C in a constant temperature freezer. When stored under these conditions the reagents are stable for 1 year.
For short term storage the qPCR MasterMix Plus for SYBR® Green I No ROX can be stored at 4 °C to 6 °C for 1 month.
The qPCR MasterMix Plus for SYBR® Green I reaction buffer should be protected from light whenever possible.
For long term storage the Reverse Transcriptase Core kit should be stored at -65 °C to -75 °C in a constant temperature freezer. When stored under these conditions the reagents are stable for 1 year.
For short term storage the Reverse Transcriptase Core kit can be stored at -15 °C to -25 °C for 6 months.
Our Two step RT qPCR MasterMixf or SYBR® Green I No ROX, will allow you to achieve reliable, consistent and accurate Real-Time RTqPCR results for SYBR® Green I detection. The Two step RT qPCR MasterMix for SYBR® Green I No ROX contains enough reagents for up to 600 - 25 µl reactions using the hotstart enzyme, HotGoldStar and has been validated on Mx4000®, Mx3000P®, Mx3005P®, MasterCycler® ep realplex, iCycler iQ®, My iQ®, iQ5, DNA Engine Opticon® 1, DNA Engine Opticon® 2, Chromo 4, Mini Opticon®, Quantica®, LC480. The Reverse Transcriptase Core kit contains10x buffer, dNTP mix, EuroScript, RNase Inhibitor and 25 mM MgCl2, oligo d(T) primers, random nonamers and RNase free water tubes. The qPCR MasterMix for SYBR® Green I No ROX contains enough reagents for up to 300 - 50 µl reactions using the hotstart enzyme, HotGoldStar and has been validated on Mx4000®, Mx3000P®, Mx3005P®, MasterCycler® ep realplex, iCycler iQ®, My iQ®, iQ5, DNA Engine Opticon® 1, DNA Engine Opticon® 2, Chromo 4, Mini Opticon®, Quantica®, LC480. The 2x buffer contains dNTP/dUTP, UNG, HotGoldStar, MgCl2 and stabilizers. A separate tube of SYBR® Green I is provided in the kit. An extra tube of 50 mM MgCl2 is provided in the kit to optimize MgCl2 concentration if necessary.
Legal notices
FOR RESEARCH USE ONLY - NOTICE TO PURCHASER: LIMITED LICENSE - Licensed dsDNA-Binding Dye Research Kits - Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,079,352, 5,789,224, 5,618,711, 6,127,155, 5,677,152, 5,773,258, 5,407,800, 5,322,770, 5,310,652, 5,994,056, 6,171,785, and claims outside the US corresponding to US Patent No. 4,889,818. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim (such as apparatus or system claims in US Patent No. 6,814,934) and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser’s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA. -
Cyclic-substituted unsymmetrical cyanine dyes are covered by U.S. Patents 5,436,134 and 5,658,751 and licensed to Eurogentec S.A by Molecular Probes, Inc in the direct research field.
LeafletsTDSMSDSCertificate of analysisTo obtain the Certificate of Analysis corresponding to your qPCR kit of interest please contact us via email and display the reference code of the qPCR kit and the batch number. Contact usProduct citationsGOTTAR M. et al., The Drosophila immune response against Gram-negative bacteria is mediated by a peptidoglycan recognition protein", Nature, n° 416, p. 640-644, 11 April 2002 LAURITZEN I. et al., "K+ -dependent Cerebellar Granule Neuron Apoptosis", Journal of Biological Chemistry, vol.278, n° 34, p. 32068-32076, May 2003 YANG Z. et al., "Protein Kinase B /Akt1 Regulates Placental Development and Fetal Growth", Journal of Biological Chemistry, vol.278, n° 34, p. 32124-32131, May 2003 WANG S. et al., "Histone deacetylase 1 represses the small GTPase RhoB expression in human nonsmall lung carcinoma cell line", Oncogene, n° 22, p. 6204-6213, 2003 STIEWE T. et al., "Quantitative TP73 Transcript Analysis in Hepatocellular Carcinomas", Clinical Cancer Research, Vol. 10, 626-633, January 2004 SWILLENS S. et al., "Instant evaluation of the absolute initial number of cDNA copies from a single real-time PCR curve", Nucleic Acids Research, vol. 32, n° 6, March 2004 LÖFSTEDT T. et al., "Induction of ID2 Expression by Hypoxia-inducible Factor-1", Journal of Biological Chemistry, vol. 279, n° 38, 39223-39231, September 2004
TAKEI K. et al., "Arabidopsis CYP735A1 and CYP735A2 Encode Cytokinin Hydroxylases That Catalyze the Biosynthesis of trans-Zeatin", Journal of Biological Chemistry, vol. 279, n° 40, p. 41866-41872, October 2004 FOURMESTRAUX V. et al., "Transcript Profiling Suggests That Differential Metabolic Adaptation of Mice to a High Fat Diet Is Associated with Changes in Liver to Muscle Lipid Fluxes", Journal of Biological Chemistry, vol. 279, n° 49, 50743-50753, December 2004
PIERREUX Chr. et al., "The Transcription Factor Hepatocyte Nuclear Factor-6/Onecut-1 Controls the Expression of Its Paralog Onecut-3 in Developing Mouse Endoderm", Journal of Biological Chemistry, vol. 279, n° 49, 51298-51304, December 2004 ROEBROEK A. et al., "Limited Redundancy of the Proprotein Convertase Furin in Mouse Liver", Journal of Biological Chemistry, vol. 279, n° 51, 53442-53450, December 2004 BAETZ A. et al., "Suppressor of Cytokine Signaling (SOCS) Proteins Indirectly Regulate Toll-like Receptor Signaling in Innate Immune Cells", Journal of Biological Chemistry, vol. 279, n° 52, 54708-54715, December 2004 FERRARI S. et al., "Chromatin Domain Boundaries Delimited by a Histone-binding Protein in Yeast", Journal of Biological Chemistry, vol. 279, n° 53, 55520-55530, December 2004 VAN LEEUWEN D. M. et al., "Differential Gene Expression in Human Peripheral Blood Mononuclear Cells Induced by Cigarette Smoke and Its Constituents", Toxicological Sciences, 86(1), 200-210, April 2005 DEKAIRELLE A.-F. et al., "Assessment of the Transcriptional Activity of p53 Improves the Prediction of Recurrence in Superficial Transitional Cell Carcinoma of the Bladder", Clinical Cancer Research, vol. 11, 4724-4732, July 1, 2005
EBNER K. et al., "Molecular Detection and Quantitative Analysis of the Entire Spectrum of Human Adenoviruses by a Two-Reaction Real-Time PCR Assay", Journal of Clinical Microbiology, vol. 43, n° 7, p. 3049-3053, July 2005 Ishibashi Y. et al., "Regulation of sialyl-Lewis x epitope expression by TNF-alpha and EGF in an airway carcinoma cell line", Glycoconjugate Journal, 22(1-2):53-62, February 2005 KUROSAWA Y. et al., "Novel method to examine hepatocyte-specific gene expression in a functional coculture system", Tissue Engineering, 11(11-12):1650-7, Nov-Dec 2005 GLASS J. et al., "Essential genes of a minimal bacterium", PNAS, vol. 103, no. 2, 425-430, January 10, 2006
SCHOLZ B. et al., "Sex-dependent gene expression in early brain development of chicken embryos", BMC Neuroscience, 15;7:12, February 2006 DECOUSSER J.-W. et al., "New Real-Time PCR Assay Using Locked Nucleic Acid Probes To Assess Prevalence of ParC Mutations in Fluoroquinolone-Susceptible Streptococcus pneumoniae Isolates from France", Antimicrobial Agents and Chemotherapy, p. 1594-1598, Vol. 50, No. 4, April 2006
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