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Wavelength-shifting Molecular Beacons

Life Science Molecular Diagnostics GMP BioManufacturing
Wavelength-shifting ... Build & Order
Eurogentec is a licensed supplier of Molecular Beacons, and offers a large number of fluorescent reporters and quenchers. All Molecular Beacons are provided double-HPLC purified. The length of Molecular Beacons with a 3’ DABCYL should be comprised between 25 to 45 bases.


Name Synthesis scale Recommended Purification* Included Purification Reference EUR
WS Molecular Beacon ROX / FAM FRET 200 nmol N/A Dual HPLC (RP+RP) PB-WB016-020 On Request
WS Molecular Beacon TAMRA / FAM FRET 200 nmol N/A Dual HPLC (RP+RP) PB-WB100-020 On Request
WS Molecular Beacon Texas Red® / FAM FRET 200 nmol N/A Dual HPLC (RP+RP) PB-WB030-020 On Request
* More info on the Purifications page

Quality Control

   MALDI-TOF Mass Spectrometry and Analytical HPLC

Delivery times

   15-39 bases: 12 Working days
                             40-80 bases: 15 Working days

Packaging

   Lyophilised

Shipping conditions

   Room temperature

Storage conditions

   -20 °C to -70 °C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Lyophilized DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.

Wavelength-Shifting Molecular Beacons are brighter than standard Molecular Beacons because of an enhancement in the intensity of the fluorescence of the emitter fluorophore. These probes contain a harvester fluorophore that absorbs strongly in the wavelength range of the monochromatic light source, an emitter fluorophore of the desired emission colour, and a non-fluorescent quencher. In the absence of complementary nucleic acids targets, the probes are non-fluorescent, whereas in the presence of targets, they fluoresce, not in the emission range of the harvester fluorophore that absorbs the light, but rather in the emission range of the emitter fluorophore. This shift in emission spectrum is due to the transfer of the absorbed energy from the harvester fluorophore to the emitter fluorophore by FRET which only takes place in probes that are bound to the targets. Wavelength-Shifting Molecular Beacons are substantially brighter than conventional Molecular Beacons that cannot efficiently absorb energy from the available monochromatic light source.

Eurogentec proposes Wavelength-Shifting Molecular Beacon probes either quenched with DABCYL or with our Deep Dark Quencher II.

Legal notices

   For Research Use only

Leaflets

Product citations


GIESENDORF B. et al., "Molecular beacons: a new approach for semiautomated mutation analysis", Clinical Chemistry, vol. 44, n° 3, p. 482-486, 1998


NILSSON M. et al., "Real-Time monitoring of rolling-circle amplification using a modified molecular beacon design", Nucleic Acids Research, vol. 30, n° 14, May 2002


KEHLENBACH R. et al., "In vitro analysis of nuclear mRNA export using molecular beacons for target detection", Nucleic Acids Research, vol. 31, n° 11, April 2003


TIMM J. et al., "Differential expression of iron-, carbon-, and oxygen-responsive mycobacterial genes in the lungs of chronically infected mice and tuberculosis patients", PNAS, vol. 100, n°24, p. 14321-14326, November 2003


DAWES S. et al., "Ribonucleotide Reduction in Mycobacterium tuberculosis: Function and Expression of Genes Encoding Class Ib and Class II Ribonucleotide Reductases", Infection and Immunity, vol. 71, n° 11, p. 6124-6131, November 2003


DAHL F. et al., "Circle-to-circle amplification for precise and sensitive DNA analysis", PNAS, vol. 101, n° 13, p. 4548-4553, March 2004





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  • By e-mail: order@eurogentec.com
  • By fax : +32 4 264 07 88
  • By mail
    Eurogentec S.A.
    LIEGE Science Park
    Rue du Bois Saint Jean 5
    4102 Seraing
    BELGIUM

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