Highligth the Missing link in your miRNA studies. The expression of more than 30 % of all human genes is controlled by miRNAs! Have you ever thought that such a control does not only happen at the mature level?

Get access to the ultimate miRNA precursor detection kit

It has been shown that the expression of miRNAs can be regulated at the post-transcriptional level (Ambros et al., 2003; obernosterer et al.,2006). Such differential processing of precursor miRNAs leads to tissue- and developmental-specific miRNA expression. This novel mechanism of the regulation of miRNA expression allows higher expression stringency but also an opportunity for quick regulation, thus allowing cells to respond more quickly to stimuli. In this context, looking exclusively at the level of mature miRNAs migth be not sufficient to correlate the phenotype of a cell with its miRNA signature. A much more reliable indicator corresponds to the level of precursor miRNA. Therefore, quantifying pre-miRNAs represents a powerful way of identifying new biomaker for human diseases such as cancer. The new MiRmaid miRNA precursor qRT-PCR primer set co-developed by Eurogentec is an exclusive system allowing the simple, accurate, and rapid quantification of more than 170 human precursor miRNAs: - Functionally validated kit for pre-miRNA expression level study - Ideal for validating expression profiling data obtained with mature miRNAs - Highly sensitive - start from as little as 1 µg total RNA - Rapid screening of hundreds of human, mouse and rat pre-miRNAs using specific primer sets - Fast, simple, economical assay based on SYBR® Green detection

What are microRNAs?

miRNAs (microRNAs) are small, single-stranded RNA molecules (19-24 nucleotides in length) that are generated from endogenous hairpin-forming transcripts encoded in the genomes of humans, animals, plants and viruses. Thus, miRNAs belong to the broad and constantly growing class of small non-coding RNAs including siRNA, stRNA, snoRNA and snRNA. Surprisingly, miRNAs are one of the largest gene families, consisting of hundreds or, in higher organisms, more than a thousand genes. So far, the findings from the Human Genome Project and subsequent research efforts indicate that the human genome contains at least ~450 miRNAs. Various studies indicate that the human genome might contain up to 1500 miRNA genes.


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