What type of chemistry do you use?
Eurogentec uses solid phase Fmoc chemistry rather than tBOC chemistry. Peptides synthesised by tBOC chemistry require more purification due to the harsh ...
What quality control (QC) information do you provide?
All peptides are analysed by MS to confirm the molecular weight. For peptides where a minimum purity has been requested we also run reverse-phase HPLC ...
How are the peptides supplied?
Peptides are shipped lyophilised at room temperature via express courier. ...
How should my peptide be stored?
Lyophilized peptides should be stored away from heat, light and moisture. Under these conditions lyophilised peptides are stable at room temperature for ...
How do I know if my peptide will be soluble?
The solubility of a peptide is often hard to predict and in the more difficult cases requires multiple attempts to find the best combination of solvents ...
How should I solubilize my peptide?
Peptide solubility characteristics vary strongly from one peptide to another and are very difficult to predict. Residues such as Ala, Cys, Ile, Leu, Met ...
What spacer is there on a biotinylated peptide?
The standard biotin modification does not include a spacer. Spacers are available on request. ...
How do you confirm a peptide is cyclised?
There are two types of cyclisations that can be performed: ...
Do you synthesize 'wobble' peptides?
Yes, we can synthesize wobble peptides. As there are known differences in the incorporation rates of different amino acids, we can compensate for these ...
Do you synthesize isotopically labelled peptides?
We can synthesise peptides containing non-radioactive atoms from commercially available building blocks. This includes amino acids containing 13C, 15N ...
What is gross peptide weight?
The gross peptide weight is the weight determined after weighing the peptide. This is the amount indicated on the tube. ...
What does net peptide content mean?
The gross weight of dry peptide doesn't consist of peptide only, but includes non-peptide components such as water, absorbed solvents, counter ions and ...
Difference between peptide content & peptide purity?
Peptide content is not an indication of peptide purity; these are two measurements. Purity is determined by HPLC and indicates the presence/absence of ...
How do you generally purify your peptides?
Our peptides are generally purified by preparative reversed phase HPLC, using two tri-fluoro acetic acid (TFA) modified buffers at pH 2. Buffer A is 0 ...
What is MALDI-TOF?
MALDI stands for Matrix Assisted Laser Desorption - Time of Flight. This machine is used to determine the mass of molecules present in a sample. We can ...
What are M+Na and M+K peaks in MALDI spectra?
It is very common to see Na (sodium) and K (potassium) adducts in the MALDI spectrum. The sodium and potassium comes from the water used in the peptide ...
How long should a peptide for antibody production be?
We usually use peptides that are 13 to 15 amino acids long in length, however shorter and longer peptides have been known to work. We have successfully ...
Should I block the termini of my peptides?
In order to mimic a protein's physical and chemical properties, you should request peptides that have a similar structure and charge to the protein. For ...
How should I conjugate a peptide to a carrier protein?
The most important factor is that the site of conjugation be not an internal amino acid and that the site of conjugation be specific for a single amino ...
What type of chemistry do you use?
Eurogentec uses solid phase Fmoc chemistry rather than tBOC chemistry. Peptides synthesised by tBOC chemistry require more purification due to the harsh ...
What quality control (QC) information do you provide?
All peptides are analysed by MS to confirm the molecular weight. For peptides where a minimum purity has been requested we also run reverse-phase HPLC ...
How are the peptides supplied?
Peptides are shipped lyophilised at room temperature via express courier. ...
How should my peptide be stored?
Lyophilized peptides should be stored away from heat, light and moisture. Under these conditions lyophilised peptides are stable at room temperature for ...
How do I know if my peptide will be soluble?
The solubility of a peptide is often hard to predict and in the more difficult cases requires multiple attempts to find the best combination of solvents ...
How should I solubilize my peptide?
Peptide solubility characteristics vary strongly from one peptide to another and are very difficult to predict. Residues such as Ala, Cys, Ile, Leu, Met ...
What spacer is there on a biotinylated peptide?
The standard biotin modification does not include a spacer. Spacers are available on request. ...
How do you confirm a peptide is cyclised?
There are two types of cyclisations that can be performed: ...
Do you synthesize 'wobble' peptides?
Yes, we can synthesize wobble peptides. As there are known differences in the incorporation rates of different amino acids, we can compensate for these ...
Do you synthesize isotopically labelled peptides?
We can synthesise peptides containing non-radioactive atoms from commercially available building blocks. This includes amino acids containing 13C, 15N ...
What is gross peptide weight?
The gross peptide weight is the weight determined after weighing the peptide. This is the amount indicated on the tube. ...
What does net peptide content mean?
The gross weight of dry peptide doesn't consist of peptide only, but includes non-peptide components such as water, absorbed solvents, counter ions and ...
Difference between peptide content & peptide purity?
Peptide content is not an indication of peptide purity; these are two measurements. Purity is determined by HPLC and indicates the presence/absence of ...
How do you generally purify your peptides?
Our peptides are generally purified by preparative reversed phase HPLC, using two tri-fluoro acetic acid (TFA) modified buffers at pH 2. Buffer A is 0 ...
What is MALDI-TOF?
MALDI stands for Matrix Assisted Laser Desorption - Time of Flight. This machine is used to determine the mass of molecules present in a sample. We can ...
What are M+Na and M+K peaks in MALDI spectra?
It is very common to see Na (sodium) and K (potassium) adducts in the MALDI spectrum. The sodium and potassium comes from the water used in the peptide ...
How long should a peptide for antibody production be?
We usually use peptides that are 13 to 15 amino acids long in length, however shorter and longer peptides have been known to work. We have successfully ...
Should I block the termini of my peptides?
In order to mimic a protein's physical and chemical properties, you should request peptides that have a similar structure and charge to the protein. For ...
How should I conjugate a peptide to a carrier protein?
The most important factor is that the site of conjugation be not an internal amino acid and that the site of conjugation be specific for a single amino ...