Peptide purity levels and Applications

Which purity for which application?

Eurogentec proposes a range of different purity levels to help you make the right choice for your application. A > 70 % pure peptide is usually sufficient for generating or testing antibodies. A mixture of closely related peptides is usually able to induce an immune response that will provide the required antibody. Nevertheless, the presence of organic impurities inherent to the synthesis process can be the source of adverse side effects like inflammatory or even toxic effects during the antibody production procedure. Therefore, our immunograde peptides always undergo a special treatment for the complete extraction of organic impurities and, where necessary, undergo one standard purification step. Peptides that are > 85 % or > 95 % pure are usually required in enzymology or biological activity studies. The quality of these peptides is checked by HPLC using a photodiode array detector and by Mass Spectrometry. These data are included in the peptide delivery.

Eurogentec proposes 4 levels of purity for its custom peptide synthesis services.

• SePop purified peptides – useful for screening purposes - average purity 60%
• > 70 % purity – immunograde peptides
• > 85 % purity – biochemistry grade peptides - semi-quantitative work
• > 95 % purity – high purity peptides - for quantitative results