At Eurogentec, llama or alpaca immunizations are conducted using customized protocols and antigens to produce heavy chain only antibodies.
Serum samples are available for testing or for IgG’s isolation.
5 – 10 weeks*
The program can be stopped at this step and you receive unpurified pAb (blood or serum).
Lymphocytes isolation & mRNA extraction
Blood lymphocytes necessary for VHH production are poorly stable.
So we provide the service for lymphocyte isolation from a blood sample (+/- 250 mL) and the RNA extraction.
The program can be stopped at this step and you receive mRNA.
VHH screening & production
At QVQ, VHH phage display libraries are generated to screen for high affinity monoclonal VHH.
- Step 1 : Two libraries with size >107 different clones and > 90% insert.
- Step 2 : The sequences of at least 6 binders from 2 families**.
- Step 3 : 0.5 mg protein of the lead clones produced in E.coli and purified (>80%).
** When the clone diversity allows
VHH antibody fragments
These are the smallest naturally occurring antibody binding domains presents many advantages and hence are considered as very promising candidates for novel therapy.
Anti-llama secondary antibodies
We provide labeled (biotin, R-PE or custom labeled) and unlabeled secondary antibodies to target llama IgG1 or IgG2/3 immunoglobulins with high precision and sensitivity.
We also offer a unique top-quality secondary antibody targeting VHH’s. This rabbit polyclonal is broad VHH-specific, as it binds to and allows the detection of VHHs from different germline sequence families.
Eurogentec and QVQ ensure that your project and your data will be handled under full confidentiality and will never be shared with external parties. Non-disclosure agreements can be executed.