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DNA = ACGT   RNA = (ACGU)   2'-O-Me RNA = [ACGU]   2'-O-MOE RNA = <ACGT>   LNA = {ACGT}   Phosphorothioates = A*C*(G*U*C)

Please note:

  • insert the letter X to introduce abasic internal modifications or modifications not listed under A, C, G, T or (U).
  • Modifications must be defined in the "Manage modifications" frame.
  • You can choose up to 2 different internal modifications by sequence.

Click on the chemistry symbol to differentiate chemistry

(default is DNA)

2' O-Me RNA

Click on the letter to add DNA/RNA bases in the sequence


Click on the letter to add Wobbles

(we will incorporate T or U during production according to the chemistry symbol)


Click on the letter X to add non-natural bases or abasic internal modifications

Internal Modification

Click on the symbol to add Linkages

(default is phosphodiester)


Manage modifications

Need help to choose your Dye and Quencher Pair ? Consult our Compatibility table

Add internal modifications:
When applicable, choose your internal modification by clicking on the black triangle ( empty-box ) related to the base to modify.

Modified bases are displayed as a plain dark box ( plain-box ).

X units ( x-chemist ) must always have an internal modification.

Choose a linkage: By default bases are linked by a phosphodiester bond ().

Clicking on the link between two bases changes it to a phosphorothioate linkage (yellow) (). A * is then added to the sequence.

 (Check the free included QCs)

Complex Oligonucleotides

For any request out of the configurator settings, please contact us