Double Dye Probes

Need to order multiple custom oligos ?

Batch order

DNA = ACGT   RNA = (ACGU)   2'-O-Me RNA = [ACGU]   2'-O-MOE RNA = <ACGT>   LNA = {ACGT}


Please note:

  • insert the letter X to introduce abasic internal modifications or modifications not listed under A, C, G, T or (U).
  • Modifications must be defined in the "Manage modifications" frame.
  • You can choose up to 2 different internal modifications by sequence.

Click on the chemistry symbol to differentiate chemistry

(default is DNA)

RNA
2' O-Me RNA
LNA
2'-O-MOE RNA

Click on the letter to add DNA/RNA bases in the sequence

Adenosine
Cytosine
Guanosine
Thymidine
Uracil

Click on the letter to add Wobbles

(we will incorporate T or U during production according to the chemistry symbol)

[C,G,T/U]
[A,G,T/U]
[A,C,T/U]
[G,T/U]
[A,C]
[A,C,G,T/U]
[A,G]
[C,G]
[A,C,G]
[A,T/U]
[C,T/U]

Click on the letter X to add non-natural bases or abasic internal modifications

Internal Modification

Click on the symbol to add Linkages

(default is phosphodiester)

Phosphorothioate

Manage modifications


Need help to choose your Dye and Quencher Pair ? Consult our Compatibility table


Add internal modifications: When applicable, choose your internal modification by clicking on the black triangle ( empty-box ) related to the base to modify.

Modified bases are displayed as a plain dark box ( plain-box ).

X units ( x-chemist ) must always have an internal modification.

 ( Check the Minimum Guaranteed Yield)
Get free primers

Get 2 free primers for each Probe ordered online (Double-Dye, Molecular Beacon or MGB).
Please note : Not applicable if ordered with the batch order file.

Each primer must be 15-30 DNA bases, unmodified and RP-Cartridge–Gold purified.

The synthesis scale of the primers :

  • must be similar to the Double-Dye Probe or Molecular Beacon one (10, 40, 200 or 1000 nmol)
  • must be selected according to the MGB Probe delivered quantity as reported in the quantity table
1

Select or configure your eligible Free Primers

2 free primers already selected

The selected probe quantity doesn't meet the conditions to get free primers.

2

Your selected primers

    No free primer selected.

    Please choose or configure up to 2 primers in the left panel

Oligos beyond standard options & large quantity requests

For requests beyond the configurator options, including special modifications or larger quantities, please contact us