Oligos

2-Amino-dA - 1 modification

97.00
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  • Cat.Number : MD-NB160-IN004
  • Availability :
    In production

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Synthesis scale

While 2-amino-dA forms a very stable base pair with T containing three hydrogen bonds, the stability of the base pair with 2-thio-dT is greatly diminished. Model building suggests that steric interactions between the 2-thiol group of thymidine and the 2-amino group of adenine tilt the bases relative to each other yielding a base pair that contains only a single hydrogen bond. However, 2-thio-dT base pairs perfectly well with A. Oligonucleotides in which A has been replaced with 2-amino-dA and T with 2-thio-dT represent an excellent example of electively Binding Complementary (SBC) oligonucleotides, able to simultaneously bind to both the sense and antisense strands of a DNA or RNA duplex. SBC 20 bases annealed against a DNA 20mer target exhibited Tm values 10°C higher than the corresponding DNA-DNA hybrid, whereas the SBC-SBC hybrid yielded Tm values 30°C lower.

Specifications

Chemistry
Molecular Mass/ Weight
  • 328,22
Modification
Modification Position
  • 5'
  • IN
Storage & stability
Resuspension condition
  • - Spin the tube briefly to collect the pellet in the bottom of the tube - Add an appropriate volume of recommended buffer (refer to technical data sheet) - Allow the tube to stand a few minutes - Vortex the tube for 15 seconds
Storage Conditions
  • Dried Oligonucleotides are stable for 18 months at ambiant temperature. Oligonucleotides in solutions are stable for 24 months at -20°C. (Tolerance -20°C± 5°C. ) For more information please refer to the Reconstitution-Storage-Stability page
Activity
Usage
  • Research use
Codes
Code Nacres
  • NA.51

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