Oligos

3' 6-FAM - 1 modification

49,00
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  • Cat.Number : MD-FL001-03004
  • Availability :
    In production

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Synthesis scale

FAM is the most commonly used fluorescent dye for oligonucleotides attachment. It exhibits high quantum yields and it is compatible with most fluorescence detection equipment. Since it has decreased fluorescence below pH 7 it is typically used in the pH range 7.5 - 8.5.

There are two isomer derivatives of FAM (carboxyfluorescein): 5-FAM and 6-FAM. By default, Eurogentec uses 6-FAM for oligonucleotide labelling.

3’ 6-FAM allows effective blockage of the 3’terminus from polymerase extension as well as exonuclease digestion.

Specifications

Chemistry
Molecular Mass/ Weight
  • 569,46
Properties
Absorbance (nm)
  • 495
Emission (nm)
  • 520
Color
Modification
Modification Position
  • 3'
Storage & stability
Resuspension condition
  • - Spin the tube briefly to collect the pellet in the bottom of the tube - Add an appropriate volume of recommended buffer (refer to technical data sheet) - Allow the tube to stand a few minutes - Vortex the tube for 15 seconds
Storage Conditions
  • Dried Oligonucleotides are stable for 18 months at ambiant temperature. Oligonucleotides in solutions are stable for 24 months at -20°C. (Tolerance -20°C± 5°C. ) Fluorescent dyes should be protected from light For more information please refer to the Reconstitution-Storage-Stability page
Activity
Usage
  • Research use

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