Oligos

5' Biotin TEG - 1 modification

52,00
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  • Cat.Number : MD-BT001-05004
  • Availability :
    In production

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Synthesis scale

Biotin-labelled oligonucleotides can be used to link the oligonucleotide to streptavidin-protein conjugates, streptavidin affinity columns, or labelled streptavidin. Attachment of a biotin molecule to the 3’ terminus can also be used to prevent 3’ exonuclease digestion and 3’ extension during amplification reactions

Biotin-TEG contains a 16-atom mixed polarity spacer based on a triethylene glycol containing 4 oxygen atoms. These O-atoms confer some hydrophilic properties to the arm. This biotin phosphoramidite can be branched allowing the introduction of multiple biotins both at the 5’ or 3’ ends of the oligonucleotide. The 16-atom spacer of Biotin-TEG provides the biotin with good accessibility towards the streptavidin. In addition, the presence of a few O-atoms within the arm renders it more linear in comparison to other more hydrophobic arms when used in aqueous buffers for hybridization experiments.

Specifications

Chemistry
Spacers Sequence
  • TEG (tetraethylene glycol)
Molecular Mass/ Weight
  • 569,61
Modification
Modification Position
  • 5'
Storage & stability
Resuspension condition
  • - Spin the tube briefly to collect the pellet in the bottom of the tube - Add an appropriate volume of recommended buffer (refer to technical data sheet) - Allow the tube to stand a few minutes - Vortex the tube for 15 seconds
Storage Conditions
  • Dried Oligonucleotides are stable for 18 months at ambiant temperature. Oligonucleotides in solutions are stable for 24 months at -20°C. (Tolerance -20°C± 5°C. ) For more information please refer to the Reconstitution-Storage-Stability page
Activity
Usage
  • Research use

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