Oligos

5'-Digoxigenin - 1 modification

110,00
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  • Cat.Number : MD-CN001-05004
  • Availability :
    In production

Select specification

Synthesis scale

Like biotin, digoxigenin (DIG) is mainly used as a non-isotopic label for DNA and oligonucleotides in a wide range of applications: diagnostics, sequencing, blot applications, and in situ hybridization. The digoxigenin label can be detected with enzyme-linked immunoassays for anti-digoxigenin protein conjugates, and visualized through a chemiluminescent reaction.

Digoxigenin can be incorporated either, at both terminus, or within the sequence. Eurogentec produces these oligonucleotides by using an amino-modifier either dT, dR or C6 to create an attachment site for the digoxigenin moeity. The spacer arm between the nucleotide and the digoxigenin consists of a 12-atom chain.

Similar to the method used for biotin, we have shown that an oligonucleotide labelled with 3 digoxigenin moieties shows the best compromise between strand hybrid stability, detection limit, and probe cost. As with biotin, the best positions for these three labels are: one label at each of the termini, and one internal with at least 15 bases between the labels, or 3 at either end, each separated by HEG Spacer.

dR-Digoxigenin (deoxyribose-Digoxigenin) modification also allows the synthesis of oligonucleotides containing successive molecules of digoxigenin anywhere in the sequence or on either terminus. Each label is linked to the deoxyribose via a 6-carbon atoms spacer arm. Each dR-Digoxigenin can also be separated by a spacer (usually HEG).

Specifications

Chemistry
Molecular Mass/ Weight
  • 680,77
Modification
Modification Position
  • 5'
Post Synthesis Coupling
  • True
Storage & stability
Resuspension condition
  • - Spin the tube briefly to collect the pellet in the bottom of the tube - Add an appropriate volume of recommended buffer (refer to technical data sheet) - Allow the tube to stand a few minutes - Vortex the tube for 15 seconds
Storage Conditions
  • Dried Oligonucleotides are stable for 18 months at ambiant temperature. Oligonucleotides in solutions are stable for 24 months at -20°C. (Tolerance -20°C± 5°C. ) For more information please refer to the Reconstitution-Storage-Stability page
Activity
Usage
  • Research use

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