The sequence of the Alexa 488-labeled C-rich telomere probe is constituted of CCCTAA repeats and is 5' labeled by the Alexa 488 fluorescent Dye. The sequence is specific for both human and mouse
In principle, fluorescence in situ hybridisation (FISH) should be able to provide information on telomere length of individual chromosomes. However the efficiency of DNA/RNA oligonucleotide hybridizations for telomeric repeats investigation is not sufficient. In comparison the use of PNA stabilizes PNA-DNA/RNA duplexes. When used with PNA probes, PNA-DNA hybridization is much faster within a couple of hours and results in significantly reduced background noise due to the high signal to background noise ratio.
* The PNA bases are only available in the following countries: Belgium, France, Germany, Italy, Luxembourg, Netherlands, Denmark, Ireland, United Kingdom, Greece, Portugal, Spain, Austria, Finland, Sweden, Cyprus, Czech Republic, Estonia, Hungary, Latvia, Lithuania, Malta, Poland, Slovakia, Slovenia, Bulgaria, Romania, Norway, Iceland, Bosnia, Serbia, Montenegro, Turkey, Switzerland, Israel, Russia and African countries.