Fluorescein isothiocyanate (FITC) remains one of the most popular fluorescent labeling reagents, probably due to the low cost of the material. 5-FITC and 6-FITC have very similar absorption and fluorescence spectra. However, the isomers may differ in their binding and reactivity to proteins, and the conjugates may elute under different chromatographic conditions or migrate differently in electrophoretic gels. 5-FITC is more widely used than 6-FITC isomer for protein labeling. FITC-protein conjugates are suitable for immunofluorescent staining, in situ hybridization, flow cytometry and other biological applications. The kit has all the essential components for performing the conjugation reaction and for purifying the FITC-protein conjugates. The kit is good for three conjugation reactions with each reaction, labeling up to 200 µg IgG. Note: AnaTag kits are not intended for peptide labeling.
Component A: 5-FITC Amino-reactive dye: 3 vials
Component B: Reaction buffer for pH adjustment of the conjugation reaction: 0.5 mL
Component C: Spin column to purify dye-protein conjugate: 3 pre-packed columns
Component D: DMSO solvent for preparing dye stock solution: 150 µL
Component E: Elution buffer for eluting dye-protein conjugate: 20 mL
Component F: Wash tube to hold buffer for Spin column: 3 tubes
Component G: Collect tube to collect dye-protein conjugate: 3 tubes
Storage & stability
Store component A at -20°C. Store all other components at 4°C. Protect component A from light and moisture.