Labeling-detection

AnaTag™ 5 - FITC Microscale Protein Labeling Kit - 1 kit

196,00
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  • Cat.Number : AS-72060
  • Availability :
    In production
  • Shipping conditions : Ice delivery fees must be applied

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Fluorescein isothiocyanate (FITC) remains one of the most popular fluorescent labeling reagents, probably due to the low cost of the material. 5-FITC and 6-FITC have very similar absorption and fluorescence spectra. However, the isomers may differ in their binding and reactivity to proteins, and the conjugates may elute under different chromatographic conditions or migrate differently in electrophoretic gels. 5-FITC is more widely used than 6-FITC isomer for protein labeling. FITC-protein conjugates are suitable for immunofluorescent staining, in situ hybridization, flow cytometry and other biological applications. The kit has all the essential components for performing the conjugation reaction and for purifying the FITC-protein conjugates. The kit is good for three conjugation reactions with each reaction, labeling up to 200 µg IgG.

Specifications

Packaging
Kits components
  • Component A: 5-FITC Amino-reactive dye: 3 vials Component B: Reaction buffer for pH adjustment of the conjugation reaction: 0.5 mL Component C: Spin column to purify dye-protein conjugate: 3 pre-packed columns Component D: DMSO solvent for preparing dye stock solution: 150 µL Component E: Elution buffer for eluting dye-protein conjugate: 20 mL Component F: Wash tube to hold buffer for Spin column: 3 tubes Component G: Collect tube to collect dye-protein conjugate: 3 tubes
Properties
Absorbance (nm)
  • 494
Emission (nm)
  • 519
Storage & stability
Storage Conditions
  • Store all kit components at 4°C; Keep Component A away from light and protected from moisture
Activity
Application
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

Citations

Pneumocystis mediates overexpression of antizyme inhibitor resulting in increased polyamine levels and apoptosis in alveolar macrophages.

J Biol Chem . 2020 Jul 14 ; 284(12) 8174 | DOI : 10.1074/jbc.M805787200.

  • C-P. Liao
  • et al

A comparison of the biochemical modifications caused by toxid and nontoxic protein oligomers in cells.

J Cell Mol Med . 2011 Sep 26 ; 15(10) 2106 | DOI : 10.1111/j.1582-4934.2010.01239.x.

  • M. Zampagni
  • et al