TAMRA is one of the most popular fluorophores used in various bioconjugations. 5-TAMRA is the purified single isomer of 5(6)-TAMRA. It is preferred for some complicated biological applications where reproducibility is more critical than material cost since the minor positional difference between 5-TAMRA and 6-TAMRA might affect some biological properties of the underlying conjugates. 5-TAMRA is more often used than 6-TAMRA for labeling peptides and proteins. 6-TAMRA is predominately used for labeling nucleotides and sequencing nucleic acids. The 5-TAMRA-protein conjugates are suitable for immunofluorescent staining, in situ hybridization, flow cytometry and other biological applications. The kit has all essential components for performing the conjugation reaction and for purifying the 5-TAMRA-protein conjugates. The kit is able to perform three conjugation reactions with each reaction labeling up to 200 µg IgG.
Note: AnaTag kits are not intended for peptide labeling.