Labeling-detection

AnaTag™ 5 - TAMRA Protein Labeling Kit - 1 kit

270,00
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  • Cat.Number : AS-72063
  • Availability :
    In stock
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TAMRA is one of the most popular fluorophores used in various bioconjugations. 5-TAMRA is the purified single isomer of 5(6)-TAMRA. It is preferred for some complicated biological applications where reproducibility is more critical than material cost since the minor positional difference between 5-TAMRA and 6-TAMRA might affect some biological properties of the underlying conjugates. 5-TAMRA is more often used than 6-TAMRA for labeling peptides and proteins. 6-TAMRA is predominately used for labeling nucleotides and sequencing nucleic acids. The 5-TAMRA-protein conjugates are suitable for immunofluorescent staining, in situ hybridization, flow cytometry and other biological applications. The kit has all essential components for performing the conjugation reaction and for purifying the 5-TAMRA-protein conjugates. The kit is able to perform three conjugation reactions with each reaction labeling up to 5 mg IgG.

Specifications

Packaging
Kits components
  • Component A: 5-TAMRA SE Amino-reactive dye: 3 vials Component B: Reaction buffer for pH adjustment of the conjugation reaction: 0.5 mL Component C: Spin column to purify dye-protein conjugate: 3 pre-packed columns Component D: DMSO solvent for preparing dye stock solution: 1 mL Component E: 10x Elution buffer for eluting dye-protein conjugate: 30 mL
Properties
Absorbance (nm)
  • 547
Emission (nm)
  • 574
Storage & stability
Storage Conditions
  • Store all kit components at 4°C; Keep Component A away from light and protected from moisture
Activity
Application
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use