HiLyte™ Fluor 555 acid, SE is an excellent amine-reactive fluorescent labeling dye that generates the protein conjugates that are only slightly red-shifted compared to those of Cy® 3 dyes, resulting in an optimal match to filters designed for Cy® dyes. The HiLyte™ Fluor 555-protein conjugates are very stable and suitable for immunofluorescent staining, in situ hybridization, flow cytometry and other biological applications. Additionally, our in-house data indicated that HiLyte™ Fluor 555 is superior to Cy® 3 in fluorescence polarization-based assays. The kit has all essential components for performing the conjugation reaction and for purifying the HiLyte™ Fluor 555-protein conjugate. The entire process takes only half an hour. The kit is able to perform three conjugation reactions with each reaction labeling up to 200 µg IgG. Kit Size: 3 labeling reactions (3 x 200 µg protein). Note: AnaTag kits are not intended for peptide labeling.
Component A: HiLyte™ Fluor 555 SE Amino-reactive dye: 3 vials
Component B: Reaction buffer for pH adjustment of the conjugation reaction: 0.5 mL
Component C: Spin column to purify dye-protein conjugate: 3 pre-packed columns
Component D: DMSO solvent for preparing dye stock solution: 150 µL
Component E: Elution buffer for eluting dye-protein conjugate: 20 mL
Component F: Wash tube to hold buffer for Spin column: 3 tubes
Component G: Collect tube to collect dye-protein conjugate: 3 tubes
Storage & stability
Store component A at -20°C. Store all other components at 4°C. Protect component A from light and moisture.