AnaTag™ HiLyte™ Fluor 555 Protein Labeling Kit - 1 kit

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  • Cat.Number : AS-72045
  • Availability :
    In production
  • Shipping conditions : Ice delivery fees must be applied

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HiLyte™ Fluor 555 acid, SE is an excellent amine-reactive fluorescent labeling dye that generates the protein conjugates that are only slightly red-shifted compared to those of Cy® 3 dyes, resulting in an optimal match to filters designed for Cy® dyes. The HiLyte™ Fluor 555-protein conjugates are very stable and suitable for immunofluorescent staining, in situ hybridization, flow cytometry and other biological applications. Additionally, our in-house data indicated that HiLyte™ Fluor 555 is superior to Cy® 3 in fluorescence polarization-based assays. The kit has all essential components for performing the conjugation reaction and for purifying the HiLyte™ Fluor 555-protein conjugate. The entire process takes only half an hour.The kit is able to perform three conjugation reactions with each reaction labeling up to 5 mg IgG.
Note: AnaTag kits are not intended for peptide labeling.


Kits components
  • Component A: HiLyte™ Fluor 555 SE Amino-reactive dye: 3 vials Component B: Reaction buffer for pH adjustment of the conjugation reaction: 0.5 mL Component C: Spin column to purify dye-protein conjugate: 3 pre-packed columns Component D: DMSO solvent for preparing dye stock solution: 1 mL Component E: 10x Elution buffer for eluting dye-protein conjugate: 30 mL
Absorbance (nm)
  • 553
Emission (nm)
  • 568
Storage & stability
Storage Conditions
  • Store component A at -20°C. Store all other components at 4°C. Protect component A from light and moisture.
Detection Method
Research Area
Sub-category Research Area
  • Research use
Code Nacres
  • NA.32

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