Labeling-detection

AnaTag™ HiLyte™ Fluor 750 Microscale Protein Labeling Kit - 1 kit

207,00
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  • Cat.Number : AS-72044
  • Availability :
    In production
  • Shipping conditions : Ice delivery fees must be applied

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HiLyte™ Fluor 750 acid, SE is the longest-wavelength amine-reactive HiLyte™ Fluor dye currently available. Its fluorescence emission maximum at 778 nm is well separated from commonly used far-red fluorophores such as HiLyte™ Fluor 647 or allophycocyanin (APC), facilitating multicolor analysis. With a peak excitation at ~754 nm, conjugates of HiLyte™ Fluor 750 dyes are well excited by a xenon-arc lamp or dye-pumped lasers operating in the 720–750 nm range. The HiLyte™ Fluor 750-protein conjugates are suitable for immunofluorescent staining, in situ hybridization, flow cytometry and other biological applications. The kit has all essential components for performing the conjugation reaction and for purifying the HiLyte™ Fluor 750-protein conjugates. The kit is able to perform three conjugation reactions with each reaction labeling up to 200 µg IgG.

Specifications

Packaging
Kits components
  • Component A: HiLyte™ Fluor 750 SE Amino-reactive dye: 3 vials Component B: Reaction buffer for pH adjustment of the conjugation reaction: 0.5 mL Component C: Spin column to purify dye-protein conjugate: 3 pre-packed columns Component D: DMSO solvent for preparing dye stock solution: 150 µL Component E: Elution buffer for eluting dye-protein conjugate: 20 mL Component F: Wash tube to hold buffer for Spin column: 3 tubes Component G: Collect tube to collect dye-protein conjugate: 3 tubes
Properties
Absorbance (nm)
  • 754
Emission (nm)
  • 778
Storage & stability
Storage Conditions
  • Store all kit components at 4°C; Keep Component A away from light and protected from moisture
Activity
Application
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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