Oligos

Biotin (dT) - 1 modification

100,00
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  • Cat.Number : MD-BT001-DT004
  • Availability :
    In production

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Synthesis scale

Biotin-labelled oligonucleotides can be used to link the oligonucleotide to streptavidin-protein conjugates, streptavidin affinity columns, or labelled streptavidin. Attachment of a biotin molecule to the 3’ terminus can also be used to prevent 3’ exonuclease digestion and 3’ extension during amplification reactions

dT biotin is a derivatized dT nucleotide with a biotin molecule on the C5-atom. It is therefore used in place of a thymidine. The biotin is linked to the dT via a 10 atom spacer.

Specifications

Chemistry
Molecular Mass/ Weight
  • 684,7
Modification
Modification Position
  • 3'
  • 5'
  • IN
Storage & stability
Resuspension condition
  • - Spin the tube briefly to collect the pellet in the bottom of the tube - Add an appropriate volume of recommended buffer (refer to technical data sheet) - Allow the tube to stand a few minutes - Vortex the tube for 15 seconds
Storage Conditions
  • Dried Oligonucleotides are stable for 18 months at ambiant temperature. Oligonucleotides in solutions are stable for 24 months at -20°C. (Tolerance -20°C± 5°C. ) For more information please refer to the Reconstitution-Storage-Stability page
Activity
Usage
  • Research use
Codes
Code Nacres
  • NA.51

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