Labeling-detection
Biotin-X NTA [Biotin-X nitrilotriacetic acid, tripotassium salt] - 5 mg
€190.00
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- Cat.Number : AS-60655
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Biotin-X nitrilotriacetic acid, tripotassium salt (biotin-X NTA) is an excellent reagent for detecting polyhistidine-containing biomolecules immobilized on membranes.
The NTA moiety chelates a Ni++ ion that is also chelated with His tags. The complex can than be detected using streptavidin-conjugates such as streptavidin-HRP. Biotin-X NTA can be removed from the His-tagged molecule at pH 4.8, allowing the blot to be reprobed.
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