PCR & qPCR

Diamond Taq® DNA Polymerase

99,00
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  • Cat.Number : TAQ-I021-1000
  • Availability :
    In stock

Select specification

Size

Quantity

Diamond Taq® polymerase is a highly thermostable enzyme produced and purified from recombinant Escherichia coli bacterium containing the Thermus aquaticus DNA Polymerase gene. The expressed enzyme catalyzes 5’?3’ synthesis of DNA with no detectable 3’?5’ proof reading exonuclease activity. This enzyme has the “extendase” activity allowing TA cloning.

Diamond Taq® polymerase is particularly recommended for PCR applications that require ultra low levels of bacterial & fungal DNA in Diagnostic and demanding Research fields. Visual confirmation of pipetting (inert red dye) can be included in the storage buffer, see Red Diamond Taq®.

Diamond Taq® polymerase can be ordered according to your needs:
Under IVD process (please enquire):
- Shipment on dry ice
- Hard copy CoA
- Full traceability from production, storage to shipment
- Tracking number sent to the customer the day of the shipment
- Customized Fill & Finish. On request Diamond Taq® can be produced with an activity from 5 to 5000U/µl; a glycerol level from 1 to 50 %
Under Research process:
- Shipment at room temperature
- Full traceability from production to storage of the product

Specifications

Packaging
Kits components
  • TAQ-I021-100- contains: - One tube (20 µl) of Diamond Taq® DNA Polymerase (concentration > 5 U/µl) - One tube (1 ml) of reaction buffer 10x (150 mM Tris-HCl pH 8.0 (at 25°C), 500 mM KCl and stabilizer) - One tube (1ml) of MgCl2 solution (25 mM MgCl2). TAQ-I021-1000 contains: - One tube (200 µl) of Diamond Taq® DNA Polymerase (concentration > 5 U/µl) - One bottle (6 ml) of reaction buffer 10x (150 mM Tris-HCl pH 8.0 (at 25°C), 500 mM KCl and stabilizer) - One bottle (6 ml) of MgCl2 solution (2
Chemistry
Molecular Mass/ Weight
  • approx. 95 kDa
Properties
Color
Quantity & Purity
Concentration
  • >= 5 U/µl
Purification Method
  • SDS-PAGE
Purity
  • > 98%
Storage & stability
Form
  • In solution
Storage Buffer
  • 20 mM Tris-HCl, 1 mM DTT, 0.1 mM EDTA, 0.1 M KCl, 0.5 % (v/v) Nonidet P40, 0.5 % (v/v) Tween 20, 50 % (v/v) glycerol and stabilizer, pH 8.0 (19 °C).
Storage Conditions
  • Storage at -20 °C is recommended 
Activity
Template type
  • DNA
Unit definition
  • One unit is defined as the amount of enzyme that incorporates, 10 nmoles of dNTPs into acid insoluble form in 30 minutes at 74 °C.
Usage
  • Research use
  • Diagnostics
Source
Host
Source / Species
  • Thermus aquaticus
Codes
Code Nacres
  • NA.55

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