Labeling-detection

DiI [1,1'-Dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine iodide] - 100 mg

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  • Cat.Number : AS-84711
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    In stock
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DiI, DiO, DiD and DiR dyes are a family of lipophilic fluorescent dyes which stain membranes and other lipophilic molecules. Their fluorescence highly increases when incorporated into membranes or bound to lipophilic molecules. Once applied onto cells, they diffuse laterally within the membranes to stain the entire cells. Their fluorescence is significantly increased upon membrane incorporation. This family of dyes fluoresces at distinct colors: DiI (orange), DiO (green), DiD (red) and DiR (far red); so they are adapted to multicolor imaging and flow cytometric of living cells. DiO and DiI can be used with standard FITC and TRITC filters respectively.
DiI is frequently used due to its very low cell toxicity. It is also used to label lipoproteins such as LDL and HDL.

Specifications

Chemistry
Molecular Formula
  • C59H97N2•I
Molecular Mass/ Weight
  • 961,32
Properties
Absorbance (nm)
  • 549
Emission (nm)
  • 565
Storage & stability
Form
  • Lyophilized
Resuspension condition
  • DMSO
Storage Conditions
  • -20°C desiccated and protected from light
Activity
Application
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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Citations

Nuclear atrophy of retinal ganglion cells precedes the Bax-dependent stage of apoptosis.

Invest Ophthalmol Vis Sci . 2013 Mar 11 ; 54(3) 1805 | DOI : 10.1167/iovs.11-9310.

  • K. Janssen
  • et al

Evaluation of corticospinal axon loss by fluorescent dye tracing in mice with experimental autoimmune encephalomyelitis.

J Neurosci Methods . 2007 Aug 25 ; 167(2) 191 | DOI : 10.1016/j.jneumeth.2007.08.013

  • Z. Liu
  • et al