dNTPs mixes are enzymatically produced from nucleotide monophosphates by a process of phosphorylation. This process uses highly specific enzymatic systems, which eliminate impurities and PCR inhibitors such as modified nucleotides and pyrophosphates. dNTPs are purified with preparative HPLC and are at least 99 % pure.
> dNTP mix containing all four nucleotides (dATP, dCTP, dGTP, dTTP) in a premixed solution, ready for immediate use (5 mM each).
For primer extension application add 4 µl of dNTP mix to 96 µl reaction mix to give a final concentration of 200 µM per dNTP.
Quality Control dNTPs are tested for the absence of DNase, RNase, protease and nicking activity. Each batch is validated rigorously for purity by HPLC, and tested for performance in a wide range of PCR templates, including a long-distance 20 kb functional test.