PCR & qPCR

dNTP Set 4 x 25 µmoles - 4 x 250 µl

140,00
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  • Cat.Number : NU-0020-50
  • Availability :
    In stock
  • Shipping conditions : Ice delivery fees must be applied

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Quantity

dNTPs sets are enzymatically produced from nucleotide monophosphates by a process of phosphorylation. This process uses highly specific enzymatic systems, which eliminate impurities and PCR inhibitors such as modified nucleotides and pyrophosphates. dNTPs are purified with preparative HPLC and are at least 99 % pure.

A pack of four vials, each containing one of the four nucleotides (dATP, dCTP, dGTP, dTTP) at 100 mM.

For primer extension application, prepare a dNTP mix and dilute to give a final concentration of 200 µM of each dNTP in a total 100 µl reaction mix.

Quality Control dNTPs are tested for the absence of DNase, RNase, protease and nicking activity. Each batch is validated rigorously for purity by HPLC, and tested for performance in a wide range of PCR templates, including a long-distance 20 kb functional test.

Specifications

Packaging
Kits components
  • The package contains: - One tube (250 µL) of dTTP 100 mM - One tube (250 µL) of dATP 100 mM - One tube (250 µL) of dCTP 100 mM - One tube (250 µL) of dGTP 100 mM
Chemistry
Molecular Mass/ Weight
  • dATP: 535.2 g/mol dGTP 533.2 g/mol dCTP 511.1 g/mol dTTP 505.9 g/mol
Properties
Color
Quantity & Purity
Concentration
  • 100 mM
Purification Method
  • HPLC
Purity
  • > 99 %
Storage & stability
Form
  • In solution
Storage Conditions
  • Store the dNTP Mix at -20°C or -70°C, in a constant temperature freezer. Avoid multiple freeze/ thaw cycles. For long-term usage, aliquoting is recommended.

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