dNTPs sets are enzymatically produced from nucleotide monophosphates by a process of phosphorylation. This process uses highly specific enzymatic systems, which eliminate impurities and PCR inhibitors such as modified nucleotides and pyrophosphates. dNTPs are purified with preparative HPLC and are at least 99 % pure.
A pack of four vials, each containing one of the four nucleotides (dATP, dCTP, dGTP, dTTP) at 100 mM.
For primer extension application, prepare a dNTP mix and dilute to give a final concentration of 200 µM of each dNTP in a total 100 µl reaction mix.
Quality Control dNTPs are tested for the absence of DNase, RNase, protease and nicking activity. Each batch is validated rigorously for purity by HPLC, and tested for performance in a wide range of PCR templates, including a long-distance 20 kb functional test.