The Fluorescein Passive Reference consists of an inert dye whose fluorescence is stable along the qPCR reaction, allowing the normalization of well-to-well variations that may result from pipetting errors or instrument limitations. Fluorescein allows the collection of Dynamic Well Factors from the experimental plate, which is the recommended method.
Eurogentec's Fluorescein passive reference has been specifically developed for use on Bio-Rad Real-Time thermocyclers such as iCycler iQ®, iQ5 or My iQ.
For your convenience, Eurogentec has also developed a SYBR® Green I kit already containing fluorescein in a ready-to-use MasterMix. Alternatively a separate tube of fluorescein can be ordered in case you would like to use fluorescein in combination with any of our No ROX SYBR® Green I MasterMix or Core Kits.