Peptides

Generic 3CLpro FRET peptide substrate - 0.1 mg

168.00
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  • Cat.Number : AS-65599
  • Availability :
    In stock

Quantity

The viral genome of the SARS-CoV-2 virus encodes a main protease, 3CLpro (also called nsp5 or Mpro). The proteolytic activity of 3CLpro is needed for viral replication. This peptide substrate serves as a generic peptide substrate for 3CLpro enzymes based on the site-specific cleavage sequence for many coronavirus 3CLpro enzymes. It can be used to measure the activity of the enzyme.
This is a FRET peptide where in its intact form, the fluorescence of HiLyte™ Fluor 488 is quenched by QXL® 520. Upon cleavage of the FRET peptide by the active enzyme, the increase of fluorescence can be continuously monitored at excitation/emission = 490/520 nm. With superior fluorescence quantum yield and longer emission wavelength, the HiLyte™ Fluor 488/QXL® 520 based FRET peptide has less interference from the autofluorescence of test compounds and cellular components and provides better assay sensitivity

Specifications

Chemistry
Sequence one letter code
  • Hilyte™ Fluor-488-ESATLQSGLRKAK-(QXL®-520)-NH2
Sequence three letter code
  • Hilyte™ Fluor 488-Glu-Ser-Ala-Thr-Leu-Gln-Ser-Gly-Leu-Arg-Lys-Ala-Lys-QXL® 520
Molecular Mass/ Weight
  • 2236.5
Properties
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Modification
Conjugation type
  • Double dyes
Modification Name
Conjugation
  • Conjugated
Quantity & Purity
Purity
  • ≥ 90%
Storage & stability
Form
  • Lyophilized
Storage Conditions
  • - 20 °C
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use
Source
Source / Species
  • SARS-CoV-2
Codes
Code Nacres
  • NA.26

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