HiLyte™Fluor 647 amine is a carbonyl-reactive fluorescent labeling dye that generates the conjugates that are slightly red-shifted compared to those of Cy®5 dyes, resulting in an optimal match to filters designed for Cy®5 dye. Its conjugate may have better performance than Cy®5 for fluorescence polarization-based assays. Extinction Coefficient (M-1cm-1): 250,000 Fluorescence quantum yield: 0.33 Fluorescence Life Time (ns): 1.0
Water: Prepare 10 mg/ml dye stock solution in water. Completely dissolve all dye content by vortexing. Dye solution should be prepared fresh, immediately before an experiment. Long-term storage in solution form
may reduce dye activity. Any solution containing the dye should be protected from light.