Spectrally similar to Cy®7 dye, HiLyte™Fluor 750 C2 maleimide is the longest-wavelength thiol-reactive HiLyte™Fluor dye currently available. Its fluorescence emission maximum at 778 nm is well separated from commonly used far-red fluorophores such as HiLyte™Fluor 647, HiLyte™Fluor 680 or allophycocyanin (APC), facilitating multicolor analysis. With a peak excitation at ~754 nm, conjugates of HiLyte™Fluor 750 dyes are well excited by a xenon-arc lamp or dye-pumped lasers operating in the 720–750 nm range. Extinction Coefficient (M-1cm-1): 275,000 Fluorescence quantum yield: 0.12 Fluorescence Life Time (ns): 0.7
Water or DMF: Prepare 10 mg/ml dye stock solution in water or DMF. Completely dissolve all dye content by vortexing. Dye solution should be prepared fresh, immediately before an experiment. Long-term
storage in solution form may reduce dye activity. Any solution containing the dye should be protected from light.