Histone deacetylases (HDACs) act as transcriptional repressors of genes catalyzing the removal of acetyl groups from a ε-N-acetyl lysine of histone. Sirtuin 1 (SIRT1), the human homolog of yeast Sir2 (Silent Information Regulator 2), is the most studied of the seven members of sirtuin family. SIRT1 has been implicated in several important cellular processes, including genomic stability and DNA repair, p53-mediated apoptosis, adipogenesis, and aging.
The recombinant human Sirtuin 1 (GenBank Accession #: NM_012238) with 193- 741 amino acids and GST tag at its N-terminal was expressed in E. coli. The molecular mass of the enzyme is approximately 87.2 kDa on SDS-PAGE. Its activity can be measured using enzymatic assay with a fluorogenic or FRET substrates