Protein

Human Sirtuin 2, recombinant

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  • Cat.Number : AS-72187
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    In stock
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Histone deacetylases (HDACs) act as transcriptional repressors of genes catalyzing the removal of acetyl groups from a ε-N-acetyl lysine of histone. Sirtuin 2 (SIRT2) belongs to a unique class of nicotinamide adenine dinucleotide (NAD+)-dependent deacetylases (class III HDACs) that target multiple protein substrates to execute diverse biological functions. Sirtuin 2 is the member of the family of Sir2 (Silent Information Regulator 2) proteins. Besides histones, substrates for SIRT2 also include various transcription factors, the tumor suppressor p53 and alpha-tubulin. Human SIRT2 is a cytoplasmic protein that increases in abundance during mitosis and regulates major events of cytokinesis.

The recombinant human Sirtuin 2 (GenBank Accession #: NM_030593) with 13-319 amino acids and His tag at its C-terminal was expressed in E. coli. The molecular mass of the enzyme is approximately 35.5 kDa on SDS-PAGE. Its activity can be measured using enzymatic assay with a fluorogenic or FRET substrate

Specifications

Chemistry
UniProt number
  • Q81XJ6
Molecular Mass/ Weight
  • 35.5 kDa
Modification
Conjugation
  • Unconjugated
Quantity & Purity
Concentration
  • lot dependent
Storage & stability
Form
  • In solution
Storage Buffer
  • 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, 3 mM DTT.
Storage Conditions
  • Store at -80 ºC. Avoid repeated freeze-thaw cycles.
Activity
Application
Biomarker Target
Unit definition
  • One unit of enzyme deacetylates 1 picomole of Green Sirtuin 2 substrate, per minute at pH 8.0 at 37° C.
Specificity
  • human Sirtuin 2 (GenBank Accession #: NM_030593) with 13-319 amino acids
Research Area
Sub-category Research Area
Usage
  • Research use
Source
Host
Source / Species
  • human

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