The iD Broad protein weight marker includes ten highly purified pre-stained proteins in the range of 5 to 270 kDa. To ensure proper identification of each protein and facilitate visualisation with high resolution, bands are covalently conjugated to 4 different dyes:
> 30 kDa band is red
> 50 kDa band is green
> 270 kDa band is orange
> 5 kDa, 15 kDa, 35 kDa, 65 kDa, 95 kDa, 130 kDa and 175 kDa bands are blue
These 7 blue bands can hence be detected using the iD BlueLadder Revealers, which stain the blue bands of prestained markers in western blotting via the binding of a monoclonal Ab specific to the blue stain.
The marker contains SDS and dithiothreitol, and is compatible with SDS-PAGE, but not native PAGE.