PCR & qPCR

Mu-MLV Reverse Transcriptase - 40000 U

255,00
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  • Cat.Number : ME-0125-400
  • Availability :
    In stock
  • Shipping conditions : Ice delivery fees must be applied

Quantity

Mu-MLV (Moloney Murine Leukemia Virus) generates complementary DNA strand starting preferably from single-stranded RNA in the presence of a primer, or from RNA-DNA hybrid. Mu-MLV reverse transcriptase has lower RNase H activity than AMV reverse transcriptase, which is an advantage when synthesizing cDNAs from long mRNAs. Mu-MLV lacks the 3’ ’ 5’ exonuclease activity. It is recommended to use 10 U/µg RNA for optimum reverse transcription.

Applications
This enzyme is particularly suited for long cDNA fragments in the context e.g. of generating cDNA libraries or for first strand cDNA synthesis prior to RT-PCR.

Specifications

Packaging
Kits components
  • One tube (200 µL) of Mu-MLV reverse transcriptase 40000 units (200U/µl) 5 Tubes of cDNA buffer (clear cap) 250 mM Tris-HCl (pH 8.3), 375 mM KCl, 50 mM DTT, 15 mM MgCl2
Quantity & Purity
Concentration
  • 200 U/µ
Storage & stability
Form
  • In solution
Storage Buffer
  • 50 mM Tris-HCl pH 8.3 (4 °C), 1 mM EDTA, 0.1 % Triton X-100, 0.1 M NaCl, 5 mM DTT, 50 % (v/v) Glycerol.
Storage Conditions
  • Storage at -20 °C is recommended.
Activity
Template type
  • RNA
Unit definition
  • One unit is the amount of enzyme that incorporates 1 nmole of dTTP into acid - insoluble form in 10 minutes at 37 °C using polyA-oligodT 12-18 as substrate.
Usage
  • Research use
Source
Host
Source / Species
  • Moloney Murine Leukemia Virus

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