To detect the various RNA classes in living cells, several approaches have been developed. One of these is based on the use of 2' O-Methyl RNA probes for the detection of small nuclear RNAs. 2' O-Methyl RNA probes are considered to perform better then DNA oligonucleotides because they are not only nuclease resistant, but also possess a higher affinity, increased specificity, faster hybridization kinetics, and a superior ability to bind to structured targets compared to DNA oligonucleotides.
The use of Molecular Beacons to detect RNAs in living improves signal-to-noise ratios by eliminating fluorescence signals derived from non-hybridized probe sequences.
It appears that linear 2' O-Methyl RNA Molecular Beacons are usually more suitable for specific detection of these RNAs, representing different classes of RNA, in the nuclei of living cells. Molecular Beacons result in images with improved signal-to-noise ratios, thereby leading to better detection sensitivity.
FAM - Absorbance = 495 nm, Emission = 520 nm
DABCYL : Quenching range [390-510]; Max 479 nm