Oligos

O Beacon 5' TET 3' DABCYL - 1 probe

Price on request
  • Cat.Number : PB-OB212-020
  • Availability :
    In production

Select specification

Synthesis scale

To detect the various RNA classes in living cells, several approaches have been developed. One of these is based on the use of 2' O-Methyl RNA probes for the detection of small nuclear RNAs. 2' O-Methyl RNA probes are considered to perform better then DNA oligonucleotides because they are not only nuclease resistant, but also possess a higher affinity, increased specificity, faster hybridization kinetics, and a superior ability to bind to structured targets compared to DNA oligonucleotides.
The use of Molecular Beacons to detect RNAs in living improves signal-to-noise ratios by eliminating fluorescence signals derived from non-hybridized probe sequences.
It appears that linear 2' O-Methyl RNA Molecular Beacons are usually more suitable for specific detection of these RNAs, representing different classes of RNA, in the nuclei of living cells. Molecular Beacons result in images with improved signal-to-noise ratios, thereby leading to better detection sensitivity.

TET - Absorbance = 521 nm, Emission = 536 nm
DABCYL : Quenching range [390-510]; Max 479 nm

Specifications

Properties
Absorbance (nm)
  • 521
Emission (nm)
  • 536
Quantity & Purity
Purification Method
  • DUAL HPLC (RP+RP)
Storage & stability
Form
  • Dried
Resuspension condition
  • - Spin the tube briefly to collect the pellet in the bottom of the tube - Add an appropriate volume of recommended buffer (refer to technical data sheet) - Allow the tube to stand a few minutes - Vortex the tube for 15 seconds
Storage Conditions
  • Dried Oligonucleotides are stable for 18 months at ambiant temperature. Oligonucleotides in solutions are stable for 24 months at -20°C. (Tolerance -20°C± 5°C. ) Probes should be protected from light For more information please refer to the Reconstitution-Storage-Stability page
Activity
Usage
  • Research use

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