PCR & qPCR

qPCR DNA Extraction and Inhibition Control Yakima Yellow®-BHQ1®

240,00
Check your price
  • Cat.Number : RT-SPCY-B02
  • Availability :
    In stock
  • Shipping conditions : Ice delivery fees must be applied

Select specification

Size

Quantity

Real-Time PCR assays are prone to inhibition by various substances found in many samples (clinical, soil, plant…). Carryover of reagents used for the isolation of nucleic acids can also inhibit amplification reactions. Other causes of false-negative results include target nucleic acid degradation, sample processing errors and thermocycler malfunction.
Benefits
Specific
- Does not interfere with target amplification
- Avoid amplification of endogenous genes
Sensitive
- Compatible with low copy templates
- Available with dark quencher for maximal signal-to-noise ratio
Designed for multiplex assays
- Detected in the Yakima Yellow®, VIC®, JOE channel
Eurogentec’s Sample Processing Control provide an accurate way to assess the integrity of your nucleic acid extraction and amplification assay.

SPC are labelled to Yakima Yellow or Cy5, custom labelling is available on request (custom-qPCR@eurogentec.com).

The Sample Processing Control (SPC) is spiked into samples before extraction to monitor poor extraction yield, PCR inhibition, incorrect pipetting or cycling parameters.

A given quantity of control can be spiked into samples before extraction. A relative (directly comparing samples) or an absolute (using a dilution curve of the control) quantification is performed after extraction to normalize the extraction yields of the samples.

Quantitative results of the spiked control within the template or within a reference buffer (pure water, reference template…) are compared in order to reject templates where PCR inhibition is high (low quality).

Add a dilution series of the optimised control on each plate and use it to normalise PCR efficiencies between plates (also for cycler to cycler data normalisation).

Specifications

Packaging
Kits components
  • 200 rxn contains: One tube (1100 µL) of 10 X EGT Control Mix (White cap) containing primers for the control and YY-BHQ-1® probe One tube (220 µL) of EGT Control DNA (Green Cap) containing Sample Processing Control 1000 rxn contains: Five tubes (1100 µL each) of 10 X EGT Control Mix (White cap) containing primers for the control and YY-BHQ-1® probe One tube (1100 µL) of EGT Control DNA (Blue Cap) containing Sample Processing Control
Properties
Absorbance (nm)
  • 530
Emission (nm)
  • 549
Color
Modification
Conjugation type
  • Double dyes
Modification Name
Conjugation
  • Conjugated
Storage & stability
Form
  • In solution
Storage Conditions
  • • For long-term storage, the qPCR DNA Extraction and Inhibition Control should be kept in the dark, at -20 °C in a constant temperature freezer. • For short-term storage, the qPCR DNA Extraction and Inhibition Control can be kept in the dark, at 4 °C to 6°C for one month. • The 10X SPC Mix should be protected from light whenever possible to avoid degradation of the probe. • Avoid multiple freeze-thaw cycles
Activity
Detection Method
Usage
  • Research use
Codes
Code Nacres
  • NA.55

You may also be interested in the following product(s)

Example of a tube containing a Double Dye MGB probe FAM

Double Dye MGB probe 5' 6-FAM-labelled - 1 probe

Cat.Number : PB-MGBEF-006
250,00
Image of a kit Takyon No Rox Probe Mmx dTTP 1250RXN

Takyon™ No ROX Probe 2X MasterMix dTTP

Cat.Number : UF-NPMT-C0701
364,00