qPCR Internal Positive Control (IPC) Yakima Yellow®-BHQ®-1 (50 µl)

Eurogentec’s Universal Exogenous qPCR Positive Control is an optimized internal control designed for use in hydrolysis probe-based qPCR assays. It helps distinguish true target negatives from false negatives caused by PCR inhibition, pipetting errors, or incorrect cycling parameters.

The optimized control can be spiked into samples before extraction (RT-SPCx-xxx) or before qPCR (RT-IPCx-xxx), without compromising the amplification efficiency of the target sequence. 

The optimized control does not match any sequence routinely found in laboratories, ensuring assay specificity. 

The optimized control is detected using a Yakima-Yellow® (VIC® equivalent)-BHQ-1® -labeled probe, while target templates are detected using probes emitting in other fluorescence channels (e.g. FAM).

Avoids amplification of endogenous genes.

Note: Alternatively, the Universal Exogenous qPCR Positive Control may be used in standardised conditions as extraction yield calibrator, template quality sensor or inter-run calibrator. A given quantity of control can be spiked into samples before extraction. A relative (directly comparing samples) or an absolute (using a dilution curve of the control) quantification is performed after extraction to normalize the extraction yields of the samples. Quantitative results of the spiked control within the template or within a reference buffer (pure water, reference template…) are compared in order to reject templates where PCR inhibition is high (low quality). Add a dilution series of the optimised control on each plate and use it to normalize PCR efficiencies between plates (also for cycler to cycler data normalization).

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