PCR & qPCR

Red'y'GoldStar® PCR Mix

103,00
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  • Cat.Number : PK-0064-02R
  • Availability :
    In stock
  • Shipping conditions : Ice delivery fees must be applied

Select specification

Size

Quantity

The Red'y'Gold Mix Mix kit contains all PCR reaction componants except the primers and the template. It is a ready-to-use formulation at 2x concentration. The kit contains sufficient reagents for 200 PCR reactions of 50 µl and an additional tube of MgCl2 to adjust the concentration if required.
Sample contains enough reagents for 40 reactions.

An easy-to-use solution:
- 2x MasterMix containing Taq DNA polymerase, dNTPs, MgCl2, buffer and red loading dye
- Extra tube of 25 mM MgCl2
- Just add primers and DNA

Improved assay reproducibility:
By handling watery solutions instead of pipetting 50 % glycerol Taq DNA polymerase solution, pipetting mistakes will be avoided and more reproducible results will be achieved.

Reduced risks
Minimum risk of contamination by minimizing pipetting steps.

Quality Control Each lot is tested for activity in PCR and a specific PCR product will be amplified after 35 cycles. 10 % of the PCR products are visualized on a 1.5 % agarose gel. The PCR is performed in duplex, using serial dilutions of 100 ng, 10 ng, 0.1 ng DNA per 50 µl PCR.

Specifications

Packaging
Kits components
  • Sample contains: - One tube (1 mL) of 2 X PCR Mix including Goldstar, dNTPs, MgCl2 and buffer - One tube (1 mL) of MgCl2 25 mM: Additional 25mM MgCl2 for optimization if needed 200 rxn contains: - Five tubes (1 mL) of 2 X PCR Mix including Goldstar, dNTPs, MgCl2 and buffer - One tube (1 mL) of MgCl2 25 mM: Additional 25mM MgCl2 for optimization if needed
Properties
Color
Quantity & Purity
Concentration
  • 2 X
Storage & stability
Form
  • In solution
Storage Conditions
  • Red’y’Gold Mix can be stored at -20 °C (in a constant temperature freezer) for 24 months or at 4 °C for 3months. Do not repeat more than 10 freeze/thaw cycles.
Activity
Template type
  • DNA
Unit definition
  • One unit of enzyme is defined as the amount required for incorporation of 10 nmoles of dNTPs into acid - insoluble material after 30 minutes amplification at 72 °C under the standard reaction conditions.
Usage
  • Research use

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