Assay-kits

SensoLyte® 390 ACE2 Activity Assay Kit Fluorimetric - 1 kit

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  • Cat.Number : AS-72086
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    In stock
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ACE2 or ACEH, angiotensin-converting enzyme 2, a homolog of angiotensin-converting enzyme, is a zinc metallopeptidase with specificity, tissue distribution, and function distinct from those of ACE. ACE2 may play a unique role in the renin-angiotensin system and mediate cardiovascular and renal functions.
The SensoLyte® 390 ACE2 Activity Assay Kit provides a convenient assay for high throughput screening of ACE2 enzyme inhibitors and inducers using a Mc-Ala/Dnp fluorescence resonance energy transfer (FRET) peptide. In the FRET peptide, the fluorescence of Mc-Ala is quenched by Dnp. Upon cleavage into two separate fragments by ACE2, the fluorescence of Mc-Ala is recovered, and can be monitored at excitation/emission = 330/390 nm. This assay can detect the activity of subnanogram level of ACE2. Assays are performed in a convenient 96-well microplate format.

Specifications

Packaging
Kits components
  • Component A: Mca/Dnp, ACE2 substrate, Ex/Em=330 nm/390 nm upon cleavage: 5 mM, 50µL Component B: Mca fluorescence reference standard, Ex/Em=330 nm /390 nm: 1 mM, 10 µL Component C: Assay Buffer: 20 mL Component D: Inhibitor of ACE2: 100 µM, 10 µL Component E: Stop Solution: 10 mL
Chemistry
UniProt number
  • Q9BYF1
Properties
Absorbance (nm)
  • 330
Emission (nm)
  • 390
Storage & stability
Storage Conditions
  • Store all kit components at -20°C. Protect Components A and B from light and from moisture. Components C and E can be stored at room temperature for convenience.
Activity
Application
Biomarker Target
Detection Method
Detection Limit
  • subnanogram
Research Area
Sub-category Research Area
Usage
  • Research use

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Citations

Conversion of Aβ43 to Aβ40 by the successive action of angiotensin‐converting enzyme 2 and angiotensin‐converting enzyme

J Neurosci Res . 2014 May 14 ; 92(9) 1178 | DOI : 10.1002/jnr.23404

  • S. Liu
  • et al

Gestational protein restriction increases angiotensin II production in rat lung

Biol Reprod . 2013 Mar 01 ; 88(3) 64 | DOI : 10.1095/biolreprod.112.103770

  • H. Gao
  • et al

Activities of angiotensin-converting enzymes ACE1 and ACE2 and inhibition by bioactive peptides in porcine ocular tissues

J Ocul Pharmacol Ther . 2009 Feb 01 ; 25(1) 23 | DOI : 10.1089/jop.2008.0081

  • S. Luhtala
  • et al