Assay-kits

SensoLyte® 520 Acetylcholinesterase Assay Kit Fluorimetric - 1 kit

269,00
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  • Cat.Number : AS-72242
  • Availability :
    In stock
  • Shipping conditions : Ice delivery fees must be applied

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Acetylcholinesterase (AChE) is a fast acting serine hydrolase that belongs to the carboxylesterase enzyme family. It hydrolyses acetylcholine into choline and acetic acid and is found in the synaptic cleft. This enzyme can be located in many organisms including roundworm, insects, and mammals. Alzheimers and myastenia gravis diseases are characterized by reduced acetylcholin; thus, drugs that inhibit AChE were developed to reduce disease symptoms. AChE can be inhibited by many natural substances such as alakloids and quinones and synthetic chemicals such as organophosphorous compounds that are frequently used as pesicides. Recently it was demonstrated that natural conpounds have different inhibitory activity in electric eel AChE compared to the human enzyme.
The SensoLyte 520 Acetylcholinesterase Assay Kit is a homogeneous assay that can be used to detect the activity of enzyme and for screening of AChE inhibitors. Active AChE generates fluorescence that can be monitored at excitation/emission=490/520 nm. The long wavelength fluorescence of the substrate is less interfered by the autofluorescence of components in biological samples and test compounds.

Specifications

Packaging
Kits components
  • Component A: Acetylcholinesterase Substrate: 100 µL Component B: Acetylcholinesterase, Human Recombinant: 1 µg/ml, 10 µL, 2 vials Component C: Acetylthiocholin: 100 µL Component D: Assay Buffer: 50 mL Component E: Acetylcholinesterase Inhibitor: 5 mM in DMSO, 20 µL
Chemistry
UniProt number
  • P22303
Properties
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • Store kit Components A, C, D, and E at -20 ?C. Store Component B at -80 °C. Protect Component A from light and from moisture.
Activity
Application
Biomarker Target
Detection Method
Detection Limit
  • 8 pg/ml
Research Area
Sub-category Research Area
Usage
  • Research use

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